Silibinin Promotes Cell Proliferation Through Facilitating G1/S Transitions by Activating Drp1-Mediated Mitochondrial Fission in Cells

Yanting You, Liqian Chen, Yifen Wu, Ming Wang, Hanqi Lu, Xinghong Zhou, Huaxi Liu, Zixuan Fu, Qiuxing He, Jinying Ou, Xiuqiong FU, Yanyan Liu, Hiu Yee KWAN, Donghui Liang, Xiaoshan Zhao*, Jiaojiao Dai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Heart, liver, and kidney, which are known as the essential organs for metabolism, possess the unique ability to regulate the proliferation function of the body against injury. Silibinin (SB), a natural polyphenolic flavonoid extracted from traditional herb Silybum marianum L., has been used to protect hepatocytes. Whether SB can regulate mitochondrial fission in normal cells and the underlying mechanisms remain unclear. Here, we showed that SB markedly promoted cell proliferation by facilitating G1/S transition via activating dynamin-related protein 1 (Drp1), which in turn mediated mitochondrial fission in these normal cells. SB dose-dependently increased the mitochondrial mass, mtDNA copy number, cellular adenosine triphosphate production, mitochondrial membrane potential, and reactive oxygen species in normal cells. Furthermore, SB dose-dependently increased the expression of Drp1. Blocking Drp1 abolished SB-induced mitochondrial fission. In conclusion, we demonstrate that SB promotes cell proliferation through facilitating G1/S transition by activating Drp1-mediated mitochondrial fission. This study suggests that SB is a potentially useful herbal derivative for the daily prevention of various diseases caused by impaired mitochondrial fission.

Original languageEnglish
JournalCell Transplantation
Volume29
DOIs
Publication statusPublished - 2020

Scopus Subject Areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

User-Defined Keywords

  • dynamin-related protein 1
  • G1/S cell cycle
  • mitochondrial fission
  • silibinin

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