Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells

Kar Wah Leung, Yuen Kit CHENG, Nai Ki MAK, Kelvin K.C. Chan, T. P. David Fan, Ricky N S WONG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

107 Citations (Scopus)

Abstract

We here provide definitive evidence that ginsenoside-Rg1, the pharmacologically active component of ginseng, is a functional ligand of the glucocorticoid receptor (GR) as determined by fluorescence polarization assay. Rg1 increased the phosphorylation of GR, phosphatidylinositol-3 kinase (PI3K), Akt/PKB and endothelial nitric oxide synthase (eNOS) leading to increase nitric oxide (NO) production in human umbilical vein endothelial cell. Rg1-induced eNOS phosphorylation and NO production were significantly reduced by RU486, LY294,002, or SH-6. Also, knockdown of GR completely eliminated the Rg1-induced NO production. This study revealed that Rg1 can indeed serve as an agonist ligand for GR and the activated GR can induce rapid NO production from eNOS via the non-transcriptional PI3K/Akt pathway.

Original languageEnglish
Pages (from-to)3211-3216
Number of pages6
JournalFEBS Letters
Volume580
Issue number13
DOIs
Publication statusPublished - 29 May 2006

Scopus Subject Areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

User-Defined Keywords

  • Endothelial cells
  • Ginsenoside-Rg1
  • Glucocorticoid receptor
  • Nitric oxide

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