Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells

Kar Wah Leung; Yuen Kit Cheng; Nai Ki Mak; Kelvin K.C. Chan; T. P. David Fan; Ricky N.S. Wong

doi:10.1016/j.febslet.2006.04.080

Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells

Kar Wah Leung, Yuen Kit Cheng, Nai Ki Mak, Kelvin K.C. Chan, T. P. David Fan, Ricky N.S. Wong^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

118 Citations (Scopus)

Abstract

We here provide definitive evidence that ginsenoside-Rg1, the pharmacologically active component of ginseng, is a functional ligand of the glucocorticoid receptor (GR) as determined by fluorescence polarization assay. Rg1 increased the phosphorylation of GR, phosphatidylinositol-3 kinase (PI3K), Akt/PKB and endothelial nitric oxide synthase (eNOS) leading to increase nitric oxide (NO) production in human umbilical vein endothelial cell. Rg1-induced eNOS phosphorylation and NO production were significantly reduced by RU486, LY294,002, or SH-6. Also, knockdown of GR completely eliminated the Rg1-induced NO production. This study revealed that Rg1 can indeed serve as an agonist ligand for GR and the activated GR can induce rapid NO production from eNOS via the non-transcriptional PI3K/Akt pathway.

Original language	English
Pages (from-to)	3211-3216
Number of pages	6
Journal	FEBS Letters
Volume	580
Issue number	13
DOIs	https://doi.org/10.1016/j.febslet.2006.04.080
Publication status	Published - 29 May 2006

Scopus Subject Areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

User-Defined Keywords

Endothelial cells
Ginsenoside-Rg1
Glucocorticoid receptor
Nitric oxide

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10.1016/j.febslet.2006.04.080

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title = "Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells",

abstract = "We here provide definitive evidence that ginsenoside-Rg1, the pharmacologically active component of ginseng, is a functional ligand of the glucocorticoid receptor (GR) as determined by fluorescence polarization assay. Rg1 increased the phosphorylation of GR, phosphatidylinositol-3 kinase (PI3K), Akt/PKB and endothelial nitric oxide synthase (eNOS) leading to increase nitric oxide (NO) production in human umbilical vein endothelial cell. Rg1-induced eNOS phosphorylation and NO production were significantly reduced by RU486, LY294,002, or SH-6. Also, knockdown of GR completely eliminated the Rg1-induced NO production. This study revealed that Rg1 can indeed serve as an agonist ligand for GR and the activated GR can induce rapid NO production from eNOS via the non-transcriptional PI3K/Akt pathway.",

keywords = "Endothelial cells, Ginsenoside-Rg1, Glucocorticoid receptor, Nitric oxide",

author = "Leung, {Kar Wah} and Cheng, {Yuen Kit} and Mak, {Nai Ki} and Chan, {Kelvin K.C.} and {David Fan}, {T. P.} and Wong, {Ricky N.S.}",

note = "Funding Information: The present work was supported by an Earmarked Research Grant (HKBU 2171/03M) of the Research Grant Council, Hong Kong SAR Government.",

year = "2006",

month = may,

day = "29",

doi = "10.1016/j.febslet.2006.04.080",

language = "English",

volume = "580",

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journal = "FEBS Letters",

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T1 - Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells

AU - Leung, Kar Wah

AU - Cheng, Yuen Kit

AU - Mak, Nai Ki

AU - Chan, Kelvin K.C.

AU - David Fan, T. P.

AU - Wong, Ricky N.S.

N1 - Funding Information: The present work was supported by an Earmarked Research Grant (HKBU 2171/03M) of the Research Grant Council, Hong Kong SAR Government.

PY - 2006/5/29

Y1 - 2006/5/29

N2 - We here provide definitive evidence that ginsenoside-Rg1, the pharmacologically active component of ginseng, is a functional ligand of the glucocorticoid receptor (GR) as determined by fluorescence polarization assay. Rg1 increased the phosphorylation of GR, phosphatidylinositol-3 kinase (PI3K), Akt/PKB and endothelial nitric oxide synthase (eNOS) leading to increase nitric oxide (NO) production in human umbilical vein endothelial cell. Rg1-induced eNOS phosphorylation and NO production were significantly reduced by RU486, LY294,002, or SH-6. Also, knockdown of GR completely eliminated the Rg1-induced NO production. This study revealed that Rg1 can indeed serve as an agonist ligand for GR and the activated GR can induce rapid NO production from eNOS via the non-transcriptional PI3K/Akt pathway.

AB - We here provide definitive evidence that ginsenoside-Rg1, the pharmacologically active component of ginseng, is a functional ligand of the glucocorticoid receptor (GR) as determined by fluorescence polarization assay. Rg1 increased the phosphorylation of GR, phosphatidylinositol-3 kinase (PI3K), Akt/PKB and endothelial nitric oxide synthase (eNOS) leading to increase nitric oxide (NO) production in human umbilical vein endothelial cell. Rg1-induced eNOS phosphorylation and NO production were significantly reduced by RU486, LY294,002, or SH-6. Also, knockdown of GR completely eliminated the Rg1-induced NO production. This study revealed that Rg1 can indeed serve as an agonist ligand for GR and the activated GR can induce rapid NO production from eNOS via the non-transcriptional PI3K/Akt pathway.

KW - Endothelial cells

KW - Ginsenoside-Rg1

KW - Glucocorticoid receptor

KW - Nitric oxide

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U2 - 10.1016/j.febslet.2006.04.080

DO - 10.1016/j.febslet.2006.04.080

M3 - Journal article

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AN - SCOPUS:33646556664

SN - 0014-5793

VL - 580

SP - 3211

EP - 3216

JO - FEBS Letters

JF - FEBS Letters

IS - 13

ER -

Signaling pathway of ginsenoside-Rg1 leading to nitric oxide production in endothelial cells

Abstract

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