Shoot-supplied ammonium targets the root auxin influx carrier AUX1 and inhibits lateral root emergence in Arabidopsis

Baohai Li, Qing Li, Yanhua Su, Hao Chen, Liming Xiong, Guohua Mi, Herbert J. Kronzucker, Weiming Shi*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

81 Citations (Scopus)

Abstract

Deposition of ammonium (NH4+) from the atmosphere is a substantial environmental problem. While toxicity resulting from root exposure to NH4+ is well studied, little is known about how shoot-supplied ammonium (SSA) affects root growth. In this study, we show that SSA significantly affects lateral root (LR) development. We show that SSA inhibits lateral root primordium (LRP) emergence, but not LRP initiation, resulting in significantly impaired LR number. We show that the inhibition is independent of abscisic acid (ABA) signalling and sucrose uptake in shoots but relates to the auxin response in roots. Expression analyses of an auxin-responsive reporter, DR5:GUS, and direct assays of auxin transport demonstrated that SSA inhibits root acropetal (rootward) auxin transport while not affecting basipetal (shootward) transport or auxin sensitivity of root cells. Mutant analyses indicated that the auxin influx carrier AUX1, but not the auxin efflux carriers PIN-FORMED (PIN)1 or PIN2, is required for this inhibition of LRP emergence and the observed auxin response. We found that AUX1 expression was modulated by SSA in vascular tissues rather than LR cap cells in roots. Taken together, our results suggest that SSA inhibits LRP emergence in Arabidopsis by interfering with AUX1-dependent auxin transport from shoot to root.

Original languageEnglish
Pages (from-to)933-946
Number of pages14
JournalPlant, Cell and Environment
Volume34
Issue number6
DOIs
Publication statusPublished - Jun 2011
Externally publishedYes

Scopus Subject Areas

  • Physiology
  • Plant Science

User-Defined Keywords

  • ABA
  • Ammonium toxicity
  • Auxin transport
  • Root
  • Shoot-derived signal

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