Sequence Analysis of the Cloned glossy8 Gene of Maize Suggests That It May Code for a β-Ketoacyl Reductase Required for the Biosynthesis of Cuticular Waxes

Xiaojie Xu, Charles R. Dietrich, Massimo Delledonne, Yiji Xia, Tsui Jung Wen, Donald S. Robertson, Basil J. Nikolau, Patrick S. Schnable*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

76 Citations (Scopus)

Abstract

The gl8 locus of maize (Zea mays L.) was previously defined by a mutation that reduces the amount and alters the composition of seedling cuticular waxes. Sixty independently derived gl8 mutant alleles were isolated from stocks that carried the Mutator transposon system. A DNA fragment that contains a Mu8 transposon and that co-segregates with one of these alleles, gl8-Mu3142, was identified and cloned. DNA flanking the Mu8 transposon was shown via allelic cross-referencing experiments to represent the gl8 locus. The gl8 probe revealed a 1.4-kb transcript present in wild-type seedling leaves and, in lesser amounts, in other organs and at other developmental stages. The amino acid sequence deduced from an apparently full-length gl8 cDNA exhibits highly significant sequence similarity to a group of enzymes from plants, eubacteria, and mammals that catalyzes the reduction of ketones. This finding suggests that the GL8 protein probably functions as a reductase during fatty acid elongation in the cuticular wax biosynthetic pathway.

Original languageEnglish
Pages (from-to)501-510
Number of pages10
JournalPlant Physiology
Volume115
Issue number2
DOIs
Publication statusPublished - Oct 1997
Externally publishedYes

Scopus Subject Areas

  • Physiology
  • Genetics
  • Plant Science

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