Sequence analysis based on ITS1 region of nuclear ribosomal DNA of Amomum villosum and ten species of Alpinia

Chun Feng Qiao, Quan Bin Han, Zhi Li Zhao, Zheng Tao Wang, Luo Shan Xu*, Hong Xi Xu*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

6 Citations (Scopus)

Abstract

Ripe fruits of Amomum villosum Lour. are important traditional Chinese medicines and food spices. Due to similar morphological and anatomical characteristics, fruits from some Alpinia species are used clinically as substitutes or adulterants of Fructus Amomi. In this study, we determined the nrDNA ITS1 (internal transcribed space 1) sequences from Amomum villosum and ten Alpinia species, which are adulterants of Fructus Amomi. The ITS1 sequences of these species were 175-179 bp in length, and the G+C contents were 53.37-56.74%. Sequence alignment revealed that there were 55 polymorphic nucleotide sites among the tested species. The base substitution were 0.57-9.60% within the ten Aplinia species, but 17.61-21.59% between Amomum villosum and the Alpinia species. These molecular data indicated that ITS1 sequences can be used to differentiate Fructus Amomi and its substitutes or adulterants from various Alpinia species.
Original languageEnglish
Pages (from-to)142-145
Number of pages4
JournalJournal of Food and Drug Analysis
Volume17
Issue number2
DOIs
Publication statusPublished - Apr 2009

Scopus Subject Areas

  • Food Science
  • Pharmacology

User-Defined Keywords

  • Alpinia
  • Amomum villousm
  • Fructus Amomi
  • nrDNA ITS1

Fingerprint

Dive into the research topics of 'Sequence analysis based on ITS1 region of nuclear ribosomal DNA of Amomum villosum and ten species of Alpinia'. Together they form a unique fingerprint.

Cite this