Separation, structure characterization, conformation and immunomodulating effect of a hyperbranched heteroglycan from Radix Astragali

Jun Yi Yin, Ben Chung Lap Chan, Hua Yu, Iris Yuen Kam Lau, Xiao Qiang Han, Sau Wan Cheng, Chun Kwok Wong, Clara Bik San Lau, Ming Yong Xie, Kwok Pui Fung, Ping Chung Leung, Simon Q B HAN*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

A water soluble polysaccharide (RAP) was isolated and purified from Radix Astragali and its structure was elucidated by monosaccharide composition, partial acid hydrolysis and methylation analysis, and further supported by FT-IR, GC-MS and 1H and 13C NMR spectra, SEM and AFM microscopy. Its average molecular weight was 1334 kDa. It was composed of Rha, Ara, Glc, Gal and GalA in a molar ratio of 0.03:1.00:0.27:0.36:0.30. The backbone consisted of 1,2,4-linked Rhap, α-1,4-linked Glcp, α-1,4-linked GalAp6Me, β-1,3,6-linked Galp, with branched at O-4 of the 1,2,4-linked Rhap and O-3 or O-4 of β-1,3,6-linked Galp. The side chains mainly consisted of α-T-Araf and α-1,5-linked Araf with O-3 as branching points, having trace Glc and Gal. The terminal residues were T-linked Araf, T-linked Glcp and T-linked Galp. Morphology analysis showed that RAP took random coil feature. RAP exhibited significant immunomodulating effects by stimulating the proliferation of human peripheral blood mononuclear cells and enhancing its interleukin production.

Original languageEnglish
Pages (from-to)667-675
Number of pages9
JournalCarbohydrate Polymers
Volume87
Issue number1
DOIs
Publication statusPublished - 4 Jan 2012

Scopus Subject Areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

User-Defined Keywords

  • Immunomodulating effect
  • Morphology feature
  • Polysaccharide
  • Radix Astragali
  • Structure character

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