Separation of tyrosine enantiomer derivatives by capillary electrophoresis with light-emitting diode-induced fluorescence detection

Weiwen Bi, Shaorong Lei, Xiupei Yang, Zemin Xu, Hongyan Yuan, Dan Xiao*, Martin M F CHOI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

29 Citations (Scopus)

Abstract

Capillary electrophoresis (CE) coupled with fiber-optic light-emitting diode-induced fluorescence detection has been developed for the separation of tyrosine (Tyr) enantiomers. R(-)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole was used as a chiral fluorescence tagged reagent for derivatization of Tyr. The effect of pH, running buffer concentration and applied voltage on enantioselectivity has been investigated. The optimum CE conditions are 15 mmol/L borate running buffer (pH 10.5) and 14-kV applied voltage. Good reproducibility was obtained with coefficient of variation (n = 7) of migration time and peak area less than 0.2 and 2.0%, respectively. The limits of detection of d- and l-Tyr derivatives were 2.9 and 2.2 μmol/L (S/N = 3), respectively. The proposed method has been successfully applied to the determination of Tyr in a commercial amino acid oral solution.

Original languageEnglish
Pages (from-to)1167-1172
Number of pages6
JournalTalanta
Volume78
Issue number3
DOIs
Publication statusPublished - 15 May 2009

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • Capillary electrophoresis
  • Enantiomers
  • Fluorescence detection
  • Light-emitting diode
  • Oral solution
  • Tyrosine

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