Abstract
Capillary electrophoresis (CE) coupled with fiber-optic light-emitting diode-induced fluorescence detection has been developed for the separation of tyrosine (Tyr) enantiomers. R(-)-4-(3-isothiocyanatopyrrolidin-1-yl)-7-(N,N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole was used as a chiral fluorescence tagged reagent for derivatization of Tyr. The effect of pH, running buffer concentration and applied voltage on enantioselectivity has been investigated. The optimum CE conditions are 15 mmol/L borate running buffer (pH 10.5) and 14-kV applied voltage. Good reproducibility was obtained with coefficient of variation (n = 7) of migration time and peak area less than 0.2 and 2.0%, respectively. The limits of detection of d- and l-Tyr derivatives were 2.9 and 2.2 μmol/L (S/N = 3), respectively. The proposed method has been successfully applied to the determination of Tyr in a commercial amino acid oral solution.
Original language | English |
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Pages (from-to) | 1167-1172 |
Number of pages | 6 |
Journal | Talanta |
Volume | 78 |
Issue number | 3 |
DOIs | |
Publication status | Published - 15 May 2009 |
User-Defined Keywords
- Capillary electrophoresis
- Enantiomers
- Fluorescence detection
- Light-emitting diode
- Oral solution
- Tyrosine