Separation of polybrominated diphenyl ethers, polychlorinated biphenyls, polychlorinated dibenzo-p-dioxins and dibenzo-furans in environmental samples using silica gel and florisil fractionation chromatography

Hanxia Liu, Qinghua Zhang, Zongwei CAI, An Li, Yawei Wang, Guibin Jiang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

75 Citations (Scopus)

Abstract

A comprehensive procedure was established for the fractionation of solvent-extracted environmental samples into three fractions containing polybrominated diphenyl ethers (PBDEs), polychlorinated biphenyls (PCBs) and polychlorinated dibenzo-p-dioxins and dibenzo-furans (PCDD/Fs), respectively. The procedure includes multi-layer silica gel chromatography to separate PBDEs from PCBs and PCDD/Fs, and subsequent separation of PCBs from PCDD/Fs using a florisil column. The numbers of congeners involved were 16, 19, and 17, for PBDEs, PCBs, and PCDD/Fs, respectively. The separation of the three chemical groups overcomes the potential mutual interferences, which are difficult to avoid even by the use of high resolution mass spectrometer (HRMS). In addition, this procedure efficiently eliminates most interfering substances from sample matrices for the subsequent instrumental analyses. The method was validated using mixed chemical standards, and applied for the analysis of environmental samples including sediment, sewage sludge and fish tissue. All the recoveries are within the acceptable ranges specified in the USEPA standard methods.

Original languageEnglish
Pages (from-to)314-320
Number of pages7
JournalAnalytica Chimica Acta
Volume557
Issue number1-2
DOIs
Publication statusPublished - 31 Jan 2006

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

User-Defined Keywords

  • Florisil
  • HRGC/HRMS
  • PBDEs
  • PCBs
  • PCDD/Fs
  • Silica gel chromatography

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