Separation of catecholamines by microchip electrophoresis with a simple integrated laser-induced fluorescence detector

Hai Fang Li, Zongwei CAI, Jin Ming Lin*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Citations (Scopus)

Abstract

A simple microchip electrophoresis-laser-induced fluorescence device was constructed and used for separation and determination of catecholamines. On the fabricated glass chip, an extra optical fiber insertion channel, which was perpendicular and extremely close to the separation channel, was directly integrated by nothing operations more than design features on the photomask. The utilization of optical fiber to transmit the excitation light and the integration fiber channel make the fluorescence detection system simple and disposable. For electrophoresis, optimization of separation conditions was investigated for reaching high separation efficiency and sensitivity. A separation efficiency as high as 106 theoretical plate numbers could be obtained for the analytes.

Original languageEnglish
Pages (from-to)183-189
Number of pages7
JournalAnalytica Chimica Acta
Volume565
Issue number2
DOIs
Publication statusPublished - 21 Apr 2006

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

User-Defined Keywords

  • Catecholamines
  • Laser-induced fluorescence
  • Microchip electrophoresis
  • Optical fiber

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