Sensitive determination of kaempferol using carbon dots as a fluorescence probe

Lizhen Liu; Feng Feng; Man Chin Paau; Qin Hu; Yang Liu; Zezhong Chen; Yunfeng Bai; Fangfang Guo; Martin M.F. Choi

doi:10.1016/j.talanta.2015.07.004

Sensitive determination of kaempferol using carbon dots as a fluorescence probe

Lizhen Liu, Feng Feng^*, Man Chin Paau, Qin Hu, Yang Liu, Zezhong Chen, Yunfeng Bai, Fangfang Guo, Martin M.F. Choi

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

32 Citations (Scopus)

Abstract

Abstract In this study, a new sensitive and convenient method for the determination of kaempferol (Kae) based on the fluorescence quenching of fluorescent carbon dots (C-dots) was developed. The C-dots were prepared by simply mixing acetic acid, water and diphosphorus pentoxide. This green synthesis approach proceeds rapidly and gives large quantities of C-dots. The fluorescence of the C-dots decreased obviously with the increase of Kae concentration. The effect of other interfering substances on the fluorescence intensity of C-dots showed low interference. Under the optimum conditions, a linear correlation was established between fluorescence intensity ratio F_o/F and the concentration of Kae in the range of 3.5-49 μM with a detection limit (S/N=3) of 38.4 nM. The proposed method has been successfully applied to determination of Kae in xindakang tablets and human serum samples with recovery in the range of 94.6-109.0%. The C-dots could be a promising fluorescence probe for the detection of Kae owing to its low-cost production, easy operation, low cytotoxicity, and excellent biocompatibility.

Original language	English
Article number	15769
Pages (from-to)	390-397
Number of pages	8
Journal	Talanta
Volume	144
DOIs	https://doi.org/10.1016/j.talanta.2015.07.004
Publication status	Published - 11 Jul 2015

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Carbon dots
Fluorescence probe and kaempferol

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10.1016/j.talanta.2015.07.004

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title = "Sensitive determination of kaempferol using carbon dots as a fluorescence probe",

abstract = "Abstract In this study, a new sensitive and convenient method for the determination of kaempferol (Kae) based on the fluorescence quenching of fluorescent carbon dots (C-dots) was developed. The C-dots were prepared by simply mixing acetic acid, water and diphosphorus pentoxide. This green synthesis approach proceeds rapidly and gives large quantities of C-dots. The fluorescence of the C-dots decreased obviously with the increase of Kae concentration. The effect of other interfering substances on the fluorescence intensity of C-dots showed low interference. Under the optimum conditions, a linear correlation was established between fluorescence intensity ratio Fo/F and the concentration of Kae in the range of 3.5-49 μM with a detection limit (S/N=3) of 38.4 nM. The proposed method has been successfully applied to determination of Kae in xindakang tablets and human serum samples with recovery in the range of 94.6-109.0%. The C-dots could be a promising fluorescence probe for the detection of Kae owing to its low-cost production, easy operation, low cytotoxicity, and excellent biocompatibility.",

keywords = "Carbon dots, Fluorescence probe and kaempferol",

author = "Lizhen Liu and Feng Feng and {Chin Paau}, Man and Qin Hu and Yang Liu and Zezhong Chen and Yunfeng Bai and Fangfang Guo and Choi, {Martin M.F.}",

note = "Funding Information: Financial supports from the National Natural Science Foundation of China ( 21175085 and 21375083 ) and HKBU Faculty Research Grant ( FRG1/13-14/039 ) are gratefully acknowledged. We would express our sincere thanks to Ms Winnie Y.K. Wu of the Institute of Advanced Materials for taking the TEM image and Ms Silva T. Mo of the Department of Chemistry, Hong Kong Baptist University for acquiring the MALDI-TOF MS. The TEM was supported by the Special Equipment Grant from the University Grants Committee of the Hong Kong Special Administrative Region, China (Grant SEG_HKBU06 ). ",

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doi = "10.1016/j.talanta.2015.07.004",

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TY - JOUR

T1 - Sensitive determination of kaempferol using carbon dots as a fluorescence probe

AU - Liu, Lizhen

AU - Feng, Feng

AU - Chin Paau, Man

AU - Hu, Qin

AU - Liu, Yang

AU - Chen, Zezhong

AU - Bai, Yunfeng

AU - Guo, Fangfang

AU - Choi, Martin M.F.

N1 - Funding Information: Financial supports from the National Natural Science Foundation of China ( 21175085 and 21375083 ) and HKBU Faculty Research Grant ( FRG1/13-14/039 ) are gratefully acknowledged. We would express our sincere thanks to Ms Winnie Y.K. Wu of the Institute of Advanced Materials for taking the TEM image and Ms Silva T. Mo of the Department of Chemistry, Hong Kong Baptist University for acquiring the MALDI-TOF MS. The TEM was supported by the Special Equipment Grant from the University Grants Committee of the Hong Kong Special Administrative Region, China (Grant SEG_HKBU06 ).

PY - 2015/7/11

Y1 - 2015/7/11

N2 - Abstract In this study, a new sensitive and convenient method for the determination of kaempferol (Kae) based on the fluorescence quenching of fluorescent carbon dots (C-dots) was developed. The C-dots were prepared by simply mixing acetic acid, water and diphosphorus pentoxide. This green synthesis approach proceeds rapidly and gives large quantities of C-dots. The fluorescence of the C-dots decreased obviously with the increase of Kae concentration. The effect of other interfering substances on the fluorescence intensity of C-dots showed low interference. Under the optimum conditions, a linear correlation was established between fluorescence intensity ratio Fo/F and the concentration of Kae in the range of 3.5-49 μM with a detection limit (S/N=3) of 38.4 nM. The proposed method has been successfully applied to determination of Kae in xindakang tablets and human serum samples with recovery in the range of 94.6-109.0%. The C-dots could be a promising fluorescence probe for the detection of Kae owing to its low-cost production, easy operation, low cytotoxicity, and excellent biocompatibility.

AB - Abstract In this study, a new sensitive and convenient method for the determination of kaempferol (Kae) based on the fluorescence quenching of fluorescent carbon dots (C-dots) was developed. The C-dots were prepared by simply mixing acetic acid, water and diphosphorus pentoxide. This green synthesis approach proceeds rapidly and gives large quantities of C-dots. The fluorescence of the C-dots decreased obviously with the increase of Kae concentration. The effect of other interfering substances on the fluorescence intensity of C-dots showed low interference. Under the optimum conditions, a linear correlation was established between fluorescence intensity ratio Fo/F and the concentration of Kae in the range of 3.5-49 μM with a detection limit (S/N=3) of 38.4 nM. The proposed method has been successfully applied to determination of Kae in xindakang tablets and human serum samples with recovery in the range of 94.6-109.0%. The C-dots could be a promising fluorescence probe for the detection of Kae owing to its low-cost production, easy operation, low cytotoxicity, and excellent biocompatibility.

KW - Carbon dots

KW - Fluorescence probe and kaempferol

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SN - 0039-9140

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SP - 390

EP - 397

JO - Talanta

JF - Talanta

M1 - 15769

ER -

Sensitive determination of kaempferol using carbon dots as a fluorescence probe

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