Selective induction of tumor necrosis factor receptor type II gene expression by tumor necrosis factor-α in C6 glioma cells

H. Huang, H.L. Lung, K.N. Leung, D. Tsang*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

10 Citations (Scopus)

Abstract

Using reverse transcription-polymerase chain reaction (RT-PCR) technique, the levels of tumor necrosis factor receptors gene expression in C6 glioma cells upon induction with tumor necrosis factor-alpha (TNF-α) were analysed. In control cells, the level of mRNA for tumor necrosis factor receptor type II (TNF-R2; 75/80 kDa) was much lower than that of tumor necrosis factor receptor type I (TNF-R1; 55/60 kDa). Upon exposure to TNF-α, the TNF-R2 mRNA level was greatly increased, while the TNF-R1 mRNA level remained unchanged even after 48 h. The induction of TNF-R2 gene expression by TNF-α was dose-dependent and seemed to be unique to TNF-α, as IL-6 had no effect. Since TNF-R2 was reported to mediate mitogenic effect in many other cell types, it is likely that the reported proliferative effect of TNF-α on astrocytes and C6 glioma cells was mediated by this TNF receptor subtype.

Original languageEnglish
Pages (from-to)889-896
Number of pages8
JournalLife Sciences
Volume62
Issue number10
DOIs
Publication statusPublished - 30 Jan 1998
Externally publishedYes

Scopus Subject Areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

User-Defined Keywords

  • C6 glioma cells
  • Gene expression
  • Tumor necrosis factor receptors

Fingerprint

Dive into the research topics of 'Selective induction of tumor necrosis factor receptor type II gene expression by tumor necrosis factor-α in C6 glioma cells'. Together they form a unique fingerprint.

Cite this