TY - JOUR
T1 - Screening and Cellular Characterization of Genetically Encoded Voltage Indicators Based on Near-Infrared Fluorescent Proteins
AU - Monakhov, Mikhail V.
AU - Matlashov, Mikhail E.
AU - Colavita, Michelangelo
AU - Song, Chenchen
AU - Shcherbakova, Daria M.
AU - Antic, Srdjan D.
AU - Verkhusha, Vladislav V.
AU - Knöpfel, Thomas
N1 - Publisher Copyright:
© 2020 American Chemical Society.
PY - 2020/11/4
Y1 - 2020/11/4
N2 - We developed genetically encoded voltage indicators using a transmembrane voltage-sensing domain and bright near-infrared fluorescent proteins derived from bacterial phytochromes. These new voltage indicators are excited by 640 nm light and emission is measured at 670 nm, allowing imaging in the near-infrared tissue transparency window. The spectral properties of our new indicators permit seamless voltage imaging with simultaneous blue-green light optogenetic actuator activation as well as simultaneous voltage-calcium imaging when paired with green calcium indicators. Iterative optimizations led to a fluorescent probe, here termed nirButterfly, which reliably reports neuronal activities including subthreshold membrane potential depolarization and hyperpolarization as well as spontaneous spiking or electrically- and optogenetically evoked action potentials. This enables largely improved all-optical causal interrogations of physiology.
AB - We developed genetically encoded voltage indicators using a transmembrane voltage-sensing domain and bright near-infrared fluorescent proteins derived from bacterial phytochromes. These new voltage indicators are excited by 640 nm light and emission is measured at 670 nm, allowing imaging in the near-infrared tissue transparency window. The spectral properties of our new indicators permit seamless voltage imaging with simultaneous blue-green light optogenetic actuator activation as well as simultaneous voltage-calcium imaging when paired with green calcium indicators. Iterative optimizations led to a fluorescent probe, here termed nirButterfly, which reliably reports neuronal activities including subthreshold membrane potential depolarization and hyperpolarization as well as spontaneous spiking or electrically- and optogenetically evoked action potentials. This enables largely improved all-optical causal interrogations of physiology.
KW - all-optical electrophysiology
KW - biosensor
KW - Butterfly
KW - FRET
KW - GEVI
KW - iRFP
UR - http://www.scopus.com/inward/record.url?scp=85095671572&partnerID=8YFLogxK
U2 - 10.1021/acschemneuro.0c00046
DO - 10.1021/acschemneuro.0c00046
M3 - Journal article
C2 - 33063984
AN - SCOPUS:85095671572
SN - 1948-7193
VL - 11
SP - 3523
EP - 3531
JO - ACS Chemical Neuroscience
JF - ACS Chemical Neuroscience
IS - 21
ER -
