TY - JOUR
T1 - Saturated long-chain fatty acid-producing bacteria contribute to enhanced colonic motility in rats
AU - Zhao, Ling
AU - Huang, Yufen
AU - Lu, Lin
AU - Yang, Wei
AU - Huang, Tao
AU - Lin, Zesi
AU - Lin, Chengyuan
AU - Kwan, Hiuyee
AU - Wong, Hoi Leong Xavier
AU - Chen, Yang
AU - Sun, Silong
AU - Xie, Xuefeng
AU - Fang, Xiaodong
AU - Yang, Huanming
AU - Wang, Jian
AU - Zhu, Lixin
AU - Bian, Zhaoxiang
N1 - Funding Information:
This project was supported by grants from Faculty Research Grant of Hong Kong Baptist University (FRG2/15-16/001 and FRG2/16-17/003), the Research Grants Council of Hong Kong Collaborative Research Fund (C2012-15G), and Guangdong-Hong Kong Technology Cooperation Funding Scheme (2016A050503039).
Publisher copyright:
© The Author(s). 2018
PY - 2018/6/14
Y1 - 2018/6/14
N2 - Background: The gut microbiota is closely associated with gastrointestinal (GI) motility disorder, but the mechanism(s) by which bacteria interact with and affect host GI motility remains unclear. In this study, through using metabolomic and metagenomic analyses, an animal model of neonatal maternal separation (NMS) characterized by accelerated colonic motility and gut dysbiosis was used to investigate the mechanism underlying microbiota-driven motility dysfunction.Results: An excess of intracolonic saturated long-chain fatty acids (SLCFAs) was associated with enhanced bowel motility in NMS rats. Heptadecanoic acid (C17:0) and stearic acid (C18:0), as the most abundant odd- and even-numbered carbon SLCFAs in the colon lumen, can promote rat colonic muscle contraction and increase stool frequency. Increase of SLCFAs was positively correlated with elevated abundances of Prevotella, Lactobacillus, and Alistipes. Functional annotation found that the level of bacterial LCFA biosynthesis was highly enriched in NMS group. Essential synthetic genes Fabs were largely identified from the genera Prevotella, Lactobacillus, and Alistipes. Pseudo germ-free (GF) rats receiving fecal microbiota from NMS donors exhibited increased defecation frequency and upregulated bacterial production of intracolonic SLCFAs. Modulation of gut dysbiosis by neomycin effectively attenuated GI motility and reduced bacterial SLCFA generation in the colon lumen of NMS rats.Conclusions: These findings reveal a previously unknown relationship between gut bacteria, intracolonic SLCFAs, and host GI motility, suggesting the importance of SLCFA-producing bacteria in GI motility disorders. Further exploration of this relationship could lead to a precise medication targeting the gut microbiota for treating GI motility disorders.
AB - Background: The gut microbiota is closely associated with gastrointestinal (GI) motility disorder, but the mechanism(s) by which bacteria interact with and affect host GI motility remains unclear. In this study, through using metabolomic and metagenomic analyses, an animal model of neonatal maternal separation (NMS) characterized by accelerated colonic motility and gut dysbiosis was used to investigate the mechanism underlying microbiota-driven motility dysfunction.Results: An excess of intracolonic saturated long-chain fatty acids (SLCFAs) was associated with enhanced bowel motility in NMS rats. Heptadecanoic acid (C17:0) and stearic acid (C18:0), as the most abundant odd- and even-numbered carbon SLCFAs in the colon lumen, can promote rat colonic muscle contraction and increase stool frequency. Increase of SLCFAs was positively correlated with elevated abundances of Prevotella, Lactobacillus, and Alistipes. Functional annotation found that the level of bacterial LCFA biosynthesis was highly enriched in NMS group. Essential synthetic genes Fabs were largely identified from the genera Prevotella, Lactobacillus, and Alistipes. Pseudo germ-free (GF) rats receiving fecal microbiota from NMS donors exhibited increased defecation frequency and upregulated bacterial production of intracolonic SLCFAs. Modulation of gut dysbiosis by neomycin effectively attenuated GI motility and reduced bacterial SLCFA generation in the colon lumen of NMS rats.Conclusions: These findings reveal a previously unknown relationship between gut bacteria, intracolonic SLCFAs, and host GI motility, suggesting the importance of SLCFA-producing bacteria in GI motility disorders. Further exploration of this relationship could lead to a precise medication targeting the gut microbiota for treating GI motility disorders.
KW - Gastrointestinal motility disorder
KW - Gut microbiota
KW - Neonatal maternal separation
KW - Saturated long-chain fatty acids
UR - http://www.scopus.com/inward/record.url?scp=85048635528&partnerID=8YFLogxK
U2 - 10.1186/s40168-018-0492-6
DO - 10.1186/s40168-018-0492-6
M3 - Journal article
C2 - 29903041
AN - SCOPUS:85048635528
SN - 2049-2618
VL - 6
JO - Microbiome
JF - Microbiome
IS - 1
M1 - 107
ER -