Abstract
This study evaluated the combined impact of salinity (2.5, 13, and 19.3 g NaCl/L) and inoculum pretreatment (acid/alkali) on the genomic and metabolic profiles of mesophilic fermentative bacteria for hydrogen (H2) production from food waste. Experimental results revealed that acid-treated inoculum showed the highest H2 yield (201.12 ± 13.84 mL H2/g of volatile solids added) under medium salinity levels compared to other experimental conditions. A 7–56% increase in H2 yield was observed for pretreated inoculum than untreated inoculum. Genomic analysis and metabolic pattern revealed that the H2 production was mainly through Clostridial-type fermentation under medium to high salinity levels, whereas Enterococcus-type fermentation under low salinity levels. Further, the genomic analysis uncovered that phyla Firmicutes (69.71–96.81%) and genus Clostridium sensu stricto 1 (33.28–94.04%) dominated during the exponential gas production phase. Overall, this study showed the significance of inoculum pretreatment for the bioconversion of food waste at different salinity levels.
| Original language | English |
|---|---|
| Article number | 127815 |
| Number of pages | 9 |
| Journal | Bioresource Technology |
| Volume | 362 |
| Early online date | 27 Aug 2022 |
| DOIs | |
| Publication status | Published - Oct 2022 |
Scopus Subject Areas
- Bioengineering
- Waste Management and Disposal
- Environmental Engineering
- Renewable Energy, Sustainability and the Environment
User-Defined Keywords
- Dark fermentation
- Food waste
- Genomic analysis
- Hydrogen
- Organic acids
- Salinity
