Saikosaponin-d inhibits T cell activation through the modulation of PKCθ, JNK, and NF-κB transcription factor

Chung Yee Leung, Liang LIU*, Ricky N S WONG, Yao Ying Zeng, Min LI, Hua Zhou

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Citations (Scopus)

Abstract

The effects of saikosaponin-d, a triterpene saponin derived from Bupleurum falcatum L. (Umbelliferae), on the signaling pathways of T cell activation were examined. The results showed that saikosaponin-d potently suppressed both early (CD69) and late (CD71) expressions of mouse T cells stimulated with Con A or PMA. It interfered with PKCθ translocation from cytosol to membrane fraction and inhibited the phosphorylations of IκBα and JNK, but not ERK, in PMA-activated mouse T cells. Additionally, it inhibited PMA and ionomycin-stimulated IL-2 production in mouse T cells. In summary, these results indicate that the mechanism by which saikosaponin-d inhibits T cell activation would involve the suppression of CD69 and CD71 expressions and IL-2 production, and the modulation of PKC pathway through PKCθ, JNK, and NF-κB transcription factor. This may herald a novel approach for further studies of saikosaponin-d as a candidate for use in the treatment of inflammatory and autoimmune diseases.

Original languageEnglish
Pages (from-to)1920-1927
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume338
Issue number4
DOIs
Publication statusPublished - 30 Dec 2005

Scopus Subject Areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

User-Defined Keywords

  • CD69
  • CD71
  • ERK
  • IκBα
  • JNK
  • PKCθ
  • Saikosaponin-d
  • Signal transduction
  • T cell activation

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