RP-HPLC测定半枝莲药材中4种主要黄酮类成分的含量

目的：建立测定半枝莲药材中野黄芩苷、野黄芩素、木犀草素和芹菜素含量的HPLC分析方法。方法：采用C18柱，测定野黄芩苷以甲醇-0.1%醋酸水溶液（40∶60）为流动相;测定野黄芩素、木犀草素和芹菜素以乙腈-0.1%醋酸水溶液为流动相，梯度洗脱（0→40min,乙腈15%→60%）。流速为1.0mL·min-1，检测波长为335nm。结果：野黄芩苷、野黄芩素、木犀草素和芹菜素分别在0.0124~0.992μg, 0.006675~0.6675μg, 0.00484~0.484μg和0.006875~0.6875μg内呈良好的线性关系。所测10份半枝莲药材中，野黄芩苷、野黄芩素、木犀草素和芹菜素的含量范围分别为0.147%~0.592%, 0.028%~0.296%, 0·007%~0·084%和0.011%~0.217%。结论：本方法简便可行，适用于半枝莲药材中4种黄酮类成分的含量测定。

OBJECTIVE: To establish HPLC methods for the determination of scutellarin, scutellarein, luteolin and apigenin in Herba scutellariae Barbata. METHODS: C18 column was used with methanol-0.1% acetic acid solution (40:60) as the mobile phase to determine scutellarin. A C18 column was used with acetonitrile -0.1% acetic acid solution as the mobile phase to determine scutellarein, luteolin and apigenin, and a linear gradient elution from 15% to 60% acetonitrile in 40 min was applied. The flow rate was 1.0 mL·min -1, and the detection wavelength was at 335 nm. RESULTS: The ranges of calibration curve of scutellarin, scutellarein, luteolin and apigenin were 0.012 4 - 0.99 2 μg, 0.006 675 - 0.667 5 μg, 0.00 484 - 0.484 μg and 0.006 875 - 0.687 5 μg, respectively. By using the established HPLC methods, 10 samples of Herba scutellariae Barbata were analyzed. The results showed that the contents of scutellarin, scutellarein, luteolin and apigenin were 0.147% - 0.592%, 0.028% - 0.296%, 0.007% - 0.084% and 0.011% - 0.217%, respectively. CONCLUSION: A simple, effective and feasible HPLC method to determine the contents of four flavonoids is established for the quality control of Herba scutellariae Barbata.