RP-HPLC法测定狼毒中岩大戟内酯A和B的含量

目的：建立测定狼毒药材中2个二萜内酯类成分岩大戟内酯A和B的HPLC定量分析方法。方法：采用C18柱（4.6mm×250mm,5μm），乙腈-水（75:25）洗脱，流速为1.0mL·min-1，检测波长为250nm。结果：狼毒大戟和月腺大戟中普遍含有岩大戟内酯A和B,2个成分的含量范围分别为0.007%～0.043%和0.012%～0.204%。瑞香狼毒和海芋中不含有这2个成分。结论：本方法专属性强，结果准确，简便可行，适用于狼毒药材中岩大戟内酯A和B的含量测定。

Objective: To establish a method for the determination of two diterpenoide lactones jolkinolide A and jolkinolide B in Chinese herbal medicine “Lang-du” by HPLC. Methods: C18 column (4.6 mm × 250 mm 5 μm) was used with acetonitrile-water (75:25) as the mobile phase. The flow rate was 1.0 mL·min-1 and the detection wavelength was 250 nm. Results: By using the established HPLC methodnine samples of “Lang-du” were analyzed to determine the contents of jolkinolide A and B. It was showed that the contents of jolkinolide A and B ranged from 0.007% to 0.043% and 0.012% to 0.204% respectively. Among the samples the two diterpenoids were found in most of the samples of Euphorbia fischeriana and E.ebracteolata, but Stellera chamaejasme and Alocasia macrorrhiza contained neither jolkinolide A nor jolkinolide B. Conclusion: A simple effective and feasible HPLC method with good accuracy precision and reproducibility to determine the contents of two diterpenoids in “Lang-du” was established which could be applied for the quality control of this herb.