Role of UHPLC in evaluating as-synthesised ligand-protected gold nanoparticles products

Man Chin Paau, Qin Hu, Yan Zhang, Martin M F CHOI*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

4 Citations (Scopus)


Analytical separation of metal nanoparticles (NPs) remains a significant challenge as it is very useful in assessing the purity, particle size, protecting ligands and dispersity of the as-synthesised NPs products. In this paper, two liquid chromatographic methods have been developed and their efficiencies in separating samples of polydisperse gold nanoparticles protected with N-acetyl-l-cysteine ligand (NAC-AuNPs) and other ultrasmall water-soluble ligand-protected gold NPs are compared. The total elution time for analysing a NAC-AuNPs sample by ultra high-performance liquid chromatography (UHPLC) is ten times shorter than that of high-performance liquid chromatography. The major attributes of UHPLC are smaller sample volume (1-2 μL) and better separation efficiency. More importantly, our proposed UHPLC method has been successfully applied to evaluate and compare polydisperse NAC-AuNPs products synthesised with the one-phase and two-phase Brust-Schiffrin methods. The results indicate that the two-phase method would harvest AuNPs product with smaller core size and less dispersity.

Original languageEnglish
Pages (from-to)2452-2457
Number of pages6
JournalAnalytical Methods
Issue number6
Publication statusPublished - 21 Mar 2015

Scopus Subject Areas

  • Analytical Chemistry
  • Chemical Engineering(all)
  • Engineering(all)


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