Role of MicroRNA-214 in ginsenoside-Rg1-induced angiogenesis

Lai Sheung Chan; Patrick Y K YUE; Nai Ki MAK; Ricky N S WONG

doi:10.1016/j.ejps.2009.08.008

Role of MicroRNA-214 in ginsenoside-Rg1-induced angiogenesis

Lai Sheung Chan, Patrick Y K YUE, Nai Ki MAK, Ricky N S WONG^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

95 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional gene modulators. Ginsenoside-Rg1, one of the active components of ginseng, has been confirmed as an angiogenesis inducer. Using miRNA microarray analysis, a total of 17 (including miR-214) and 5 miRNAs were found to be down- or up-regulated by Rg1 in human umbilical vein endothelial cells (HUVECs), respectively. Since miR-214 is closely related to endothelial nitric oxide synthase (eNOS) and hence angiogenesis, its expression was further validated by qRT-PCR. We also investigated the role of miR-214 on eNOS expression and in tubulogenesis and motility of HUVEC by transfection of specific miRNA inhibitor or precursor. Our results suggested that Rg1 can down-regulate miR-214 expression in HUVEC, leading to an increase in eNOS expression, and in vitro cell migration and tube formation which can possibly promote angiogenesis. These results signify a new understanding towards how a simple natural compound can affect physiological changes through modulation of miRNA expression.

Original language	English
Pages (from-to)	370-377
Number of pages	8
Journal	European Journal of Pharmaceutical Sciences
Volume	38
Issue number	4
DOIs	https://doi.org/10.1016/j.ejps.2009.08.008
Publication status	Published - 5 Nov 2009

Scopus Subject Areas

Pharmaceutical Science

User-Defined Keywords

Angiogenesis
eNOS
Ginsenoside-Rg1
HUVEC
MicroRNA
miR-214

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10.1016/j.ejps.2009.08.008

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title = "Role of MicroRNA-214 in ginsenoside-Rg1-induced angiogenesis",

abstract = "MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional gene modulators. Ginsenoside-Rg1, one of the active components of ginseng, has been confirmed as an angiogenesis inducer. Using miRNA microarray analysis, a total of 17 (including miR-214) and 5 miRNAs were found to be down- or up-regulated by Rg1 in human umbilical vein endothelial cells (HUVECs), respectively. Since miR-214 is closely related to endothelial nitric oxide synthase (eNOS) and hence angiogenesis, its expression was further validated by qRT-PCR. We also investigated the role of miR-214 on eNOS expression and in tubulogenesis and motility of HUVEC by transfection of specific miRNA inhibitor or precursor. Our results suggested that Rg1 can down-regulate miR-214 expression in HUVEC, leading to an increase in eNOS expression, and in vitro cell migration and tube formation which can possibly promote angiogenesis. These results signify a new understanding towards how a simple natural compound can affect physiological changes through modulation of miRNA expression.",

keywords = "Angiogenesis, eNOS, Ginsenoside-Rg1, HUVEC, MicroRNA, miR-214",

author = "Chan, {Lai Sheung} and YUE, {Patrick Y K} and MAK, {Nai Ki} and WONG, {Ricky N S}",

note = "Funding Information: We wish to thank Dr. Timothy Yip and Mr. Cadmon Lim, Department of Clinical Oncology, Queen Elizabeth Hospital, HK, for their technical assistance in the microarray data analysis. This work was supported by the Research Grants ( HKBU 2476/06M and HKBU 1/06C ) of the Research Grant Committee, Hong Kong SAR Government and Faculty Research Grants of the Hong Kong Baptist University ( FRG2/08-09/085 ). ",

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AU - Chan, Lai Sheung

AU - YUE, Patrick Y K

AU - MAK, Nai Ki

AU - WONG, Ricky N S

N1 - Funding Information: We wish to thank Dr. Timothy Yip and Mr. Cadmon Lim, Department of Clinical Oncology, Queen Elizabeth Hospital, HK, for their technical assistance in the microarray data analysis. This work was supported by the Research Grants ( HKBU 2476/06M and HKBU 1/06C ) of the Research Grant Committee, Hong Kong SAR Government and Faculty Research Grants of the Hong Kong Baptist University ( FRG2/08-09/085 ).

PY - 2009/11/5

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N2 - MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional gene modulators. Ginsenoside-Rg1, one of the active components of ginseng, has been confirmed as an angiogenesis inducer. Using miRNA microarray analysis, a total of 17 (including miR-214) and 5 miRNAs were found to be down- or up-regulated by Rg1 in human umbilical vein endothelial cells (HUVECs), respectively. Since miR-214 is closely related to endothelial nitric oxide synthase (eNOS) and hence angiogenesis, its expression was further validated by qRT-PCR. We also investigated the role of miR-214 on eNOS expression and in tubulogenesis and motility of HUVEC by transfection of specific miRNA inhibitor or precursor. Our results suggested that Rg1 can down-regulate miR-214 expression in HUVEC, leading to an increase in eNOS expression, and in vitro cell migration and tube formation which can possibly promote angiogenesis. These results signify a new understanding towards how a simple natural compound can affect physiological changes through modulation of miRNA expression.

AB - MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional gene modulators. Ginsenoside-Rg1, one of the active components of ginseng, has been confirmed as an angiogenesis inducer. Using miRNA microarray analysis, a total of 17 (including miR-214) and 5 miRNAs were found to be down- or up-regulated by Rg1 in human umbilical vein endothelial cells (HUVECs), respectively. Since miR-214 is closely related to endothelial nitric oxide synthase (eNOS) and hence angiogenesis, its expression was further validated by qRT-PCR. We also investigated the role of miR-214 on eNOS expression and in tubulogenesis and motility of HUVEC by transfection of specific miRNA inhibitor or precursor. Our results suggested that Rg1 can down-regulate miR-214 expression in HUVEC, leading to an increase in eNOS expression, and in vitro cell migration and tube formation which can possibly promote angiogenesis. These results signify a new understanding towards how a simple natural compound can affect physiological changes through modulation of miRNA expression.

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Role of MicroRNA-214 in ginsenoside-Rg1-induced angiogenesis

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