Role of MicroRNA-214 in ginsenoside-Rg1-induced angiogenesis

Lai Sheung Chan, Patrick Y K YUE, Nai Ki MAK, Ricky N S WONG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

86 Citations (Scopus)

Abstract

MicroRNAs (miRNAs) are small non-coding RNAs that act as post-transcriptional gene modulators. Ginsenoside-Rg1, one of the active components of ginseng, has been confirmed as an angiogenesis inducer. Using miRNA microarray analysis, a total of 17 (including miR-214) and 5 miRNAs were found to be down- or up-regulated by Rg1 in human umbilical vein endothelial cells (HUVECs), respectively. Since miR-214 is closely related to endothelial nitric oxide synthase (eNOS) and hence angiogenesis, its expression was further validated by qRT-PCR. We also investigated the role of miR-214 on eNOS expression and in tubulogenesis and motility of HUVEC by transfection of specific miRNA inhibitor or precursor. Our results suggested that Rg1 can down-regulate miR-214 expression in HUVEC, leading to an increase in eNOS expression, and in vitro cell migration and tube formation which can possibly promote angiogenesis. These results signify a new understanding towards how a simple natural compound can affect physiological changes through modulation of miRNA expression.

Original languageEnglish
Pages (from-to)370-377
Number of pages8
JournalEuropean Journal of Pharmaceutical Sciences
Volume38
Issue number4
DOIs
Publication statusPublished - 5 Nov 2009

Scopus Subject Areas

  • Pharmaceutical Science

User-Defined Keywords

  • Angiogenesis
  • eNOS
  • Ginsenoside-Rg1
  • HUVEC
  • MicroRNA
  • miR-214

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