RNA helicase-like protein as an early regulator of transcription factors for plant chilling and freezing tolerance

Zhizhong Gong; Hojoung Lee; Liming Xiong; André Jagendorf; Becky Stevenson; Jian Kang Zhu

doi:10.1073/pnas.172399299

RNA helicase-like protein as an early regulator of transcription factors for plant chilling and freezing tolerance

Zhizhong Gong, Hojoung Lee, Liming Xiong, André Jagendorf, Becky Stevenson, Jian Kang Zhu^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

238 Citations (Scopus)

Abstract

Susceptibility to chilling injury prevents the cultivation of many important crops and limits the extended storage of horticultural commodities. Although freezing tolerance is acquired through cold-induced gene expression changes mediated in part by the CBF family of transcriptional activators, whether plant chilling resistance or sensitivity involves the CBF genes is not known. We report here that an Arabidopsis thaliana mutant impaired in the cold-regulated expression of CBF genes and their downstream target genes is sensitive to chilling stress. Expression of CBF3 under a strong constitutive promoter restores chilling resistance to the mutant plants. The mutated gene was cloned and found to encode a nuclear localized RNA helicase. Our results identify a regulator of CBF genes, and demonstrate the importance of gene regulation and the CBF transcriptional activators in plant chilling resistance.

Original language	English
Pages (from-to)	11507-11512
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	99
Issue number	17
DOIs	https://doi.org/10.1073/pnas.172399299
Publication status	Published - 20 Aug 2002

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title = "RNA helicase-like protein as an early regulator of transcription factors for plant chilling and freezing tolerance",

abstract = "Susceptibility to chilling injury prevents the cultivation of many important crops and limits the extended storage of horticultural commodities. Although freezing tolerance is acquired through cold-induced gene expression changes mediated in part by the CBF family of transcriptional activators, whether plant chilling resistance or sensitivity involves the CBF genes is not known. We report here that an Arabidopsis thaliana mutant impaired in the cold-regulated expression of CBF genes and their downstream target genes is sensitive to chilling stress. Expression of CBF3 under a strong constitutive promoter restores chilling resistance to the mutant plants. The mutated gene was cloned and found to encode a nuclear localized RNA helicase. Our results identify a regulator of CBF genes, and demonstrate the importance of gene regulation and the CBF transcriptional activators in plant chilling resistance.",

author = "Zhizhong Gong and Hojoung Lee and Liming Xiong and Andr{\'e} Jagendorf and Becky Stevenson and Zhu, {Jian Kang}",

note = "Funding information: We thank Dr. Julio Salinas for the desaturase gene probe and Dr. David W. Ehrhardt for pEGAD vector. This work was supported by U.S. Department of Agriculture National Research Initiative Grant 2000-00664 and National Science Foundation Grant IBN-9808398 (to J.-K.Z.). Publisher copyright: {\textcopyright} 2002, The National Academy of Sciences.",

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doi = "10.1073/pnas.172399299",

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RNA helicase-like protein as an early regulator of transcription factors for plant chilling and freezing tolerance. / Gong, Zhizhong; Lee, Hojoung; Xiong, Liming et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, No. 17, 20.08.2002, p. 11507-11512.

Research output: Contribution to journal › Journal article › peer-review

TY - JOUR

T1 - RNA helicase-like protein as an early regulator of transcription factors for plant chilling and freezing tolerance

AU - Gong, Zhizhong

AU - Lee, Hojoung

AU - Xiong, Liming

AU - Jagendorf, André

AU - Stevenson, Becky

AU - Zhu, Jian Kang

N1 - Funding information: We thank Dr. Julio Salinas for the desaturase gene probe and Dr. David W. Ehrhardt for pEGAD vector. This work was supported by U.S. Department of Agriculture National Research Initiative Grant 2000-00664 and National Science Foundation Grant IBN-9808398 (to J.-K.Z.). Publisher copyright: © 2002, The National Academy of Sciences.

PY - 2002/8/20

Y1 - 2002/8/20

N2 - Susceptibility to chilling injury prevents the cultivation of many important crops and limits the extended storage of horticultural commodities. Although freezing tolerance is acquired through cold-induced gene expression changes mediated in part by the CBF family of transcriptional activators, whether plant chilling resistance or sensitivity involves the CBF genes is not known. We report here that an Arabidopsis thaliana mutant impaired in the cold-regulated expression of CBF genes and their downstream target genes is sensitive to chilling stress. Expression of CBF3 under a strong constitutive promoter restores chilling resistance to the mutant plants. The mutated gene was cloned and found to encode a nuclear localized RNA helicase. Our results identify a regulator of CBF genes, and demonstrate the importance of gene regulation and the CBF transcriptional activators in plant chilling resistance.

AB - Susceptibility to chilling injury prevents the cultivation of many important crops and limits the extended storage of horticultural commodities. Although freezing tolerance is acquired through cold-induced gene expression changes mediated in part by the CBF family of transcriptional activators, whether plant chilling resistance or sensitivity involves the CBF genes is not known. We report here that an Arabidopsis thaliana mutant impaired in the cold-regulated expression of CBF genes and their downstream target genes is sensitive to chilling stress. Expression of CBF3 under a strong constitutive promoter restores chilling resistance to the mutant plants. The mutated gene was cloned and found to encode a nuclear localized RNA helicase. Our results identify a regulator of CBF genes, and demonstrate the importance of gene regulation and the CBF transcriptional activators in plant chilling resistance.

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JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

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ER -

RNA helicase-like protein as an early regulator of transcription factors for plant chilling and freezing tolerance

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