RNA-dependent RNA polymerase gene sequence from foot-and-mouth disease virus in Hong Kong

Xi Chen; Qian Feng; Zhiyin Wu; Yingying Liu; Kun Huang; Rui Shi; Sidi Chen; Wen Lu; Mingxiao Ding; Richard A. Collins; Yin Wan Wendy Fung; Lok Ting Lau; Albert Cheung Hoi Yu; Jianguo Chen

doi:10.1016/S0006-291X(03)01511-0

RNA-dependent RNA polymerase gene sequence from foot-and-mouth disease virus in Hong Kong

Xi Chen, Qian Feng, Zhiyin Wu, Yingying Liu, Kun Huang, Rui Shi, Sidi Chen, Wen Lu, Mingxiao Ding, Richard A. Collins, Yin Wan Wendy Fung, Lok Ting Lau, Albert Cheung Hoi Yu, Jianguo Chen^*

^*Corresponding author for this work

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9 Citations (Scopus)

Abstract

A foot-and-mouth disease virus (FMDV, HKN/2002) was isolated in Hong Kong in 2002. The nucleotide sequence of the 3D^pol gene encoding the viral RNA-dependent RNA polymerase was determined and compared with that of the same gene from other FMDVs. The 3D^pol gene was 1410 nucleotides in length encoding a protein of 470 amino acid residues. Sequence comparisons indicated that HKN/2002 belonged to serotype O. An evolutionary tree based on the 3D^pol sequences of 20 FMDV isolates revealed that the nucleotide sequence of the HKN/2002 3D^pol gene was most similar to those of isolates found in Taiwan in 1997, suggesting that they share a common ancestor. The amino acid sequence of the HKN/2002 3D^pol gene was determined and aligned with those of representative isolates from seven other Picornaviridae genera. Eight highly conserved regions were detected, indicating a conserved functional relevance for these motifs. Alignment of 20 FMDV 3D ^pol amino acid sequences revealed a hypermutation region near the N-terminus that may help the virus evade host immune systems.

Original language	English
Pages (from-to)	899-905
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	308
Issue number	4
DOIs	https://doi.org/10.1016/S0006-291X(03)01511-0
Publication status	Published - 5 Sept 2003

Scopus Subject Areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

User-Defined Keywords

3D gene
Foot-and-mouth disease virus
Hypermutation
RNA-dependent RNA polymerase
Sequence alignment
Serotype

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0006-291X(03)01511-0

Cite this

@article{0c88677db796423a84c3e62222ebb72e,

title = "RNA-dependent RNA polymerase gene sequence from foot-and-mouth disease virus in Hong Kong",

abstract = "A foot-and-mouth disease virus (FMDV, HKN/2002) was isolated in Hong Kong in 2002. The nucleotide sequence of the 3Dpol gene encoding the viral RNA-dependent RNA polymerase was determined and compared with that of the same gene from other FMDVs. The 3Dpol gene was 1410 nucleotides in length encoding a protein of 470 amino acid residues. Sequence comparisons indicated that HKN/2002 belonged to serotype O. An evolutionary tree based on the 3Dpol sequences of 20 FMDV isolates revealed that the nucleotide sequence of the HKN/2002 3Dpol gene was most similar to those of isolates found in Taiwan in 1997, suggesting that they share a common ancestor. The amino acid sequence of the HKN/2002 3Dpol gene was determined and aligned with those of representative isolates from seven other Picornaviridae genera. Eight highly conserved regions were detected, indicating a conserved functional relevance for these motifs. Alignment of 20 FMDV 3D pol amino acid sequences revealed a hypermutation region near the N-terminus that may help the virus evade host immune systems.",

keywords = "3D gene, Foot-and-mouth disease virus, Hypermutation, RNA-dependent RNA polymerase, Sequence alignment, Serotype",

author = "Xi Chen and Qian Feng and Zhiyin Wu and Yingying Liu and Kun Huang and Rui Shi and Sidi Chen and Wen Lu and Mingxiao Ding and Collins, {Richard A.} and Fung, {Yin Wan Wendy} and Lau, {Lok Ting} and Yu, {Albert Cheung Hoi} and Jianguo Chen",

note = "Funding Information: The authors thank Trevor Ellis and Pamela Li Chui Har (Agriculture, Fisheries, and Conservation Department, Veterinary Laboratory Division, Tai Lung Experimental Station, Lin Tong Mei, Sheung Shui, Hong Kong SAR, China) for providing virus samples. This work was supported by Grant G1999011904 from the Special Funds for Major State Basic Research of China to J.C. The Jun-Zheng Foundation at PKU supported X.C. Publisher copyright: {\textcopyright} 2003 Elsevier Inc. All rights reserved.",

year = "2003",

month = sep,

day = "5",

doi = "10.1016/S0006-291X(03)01511-0",

language = "English",

volume = "308",

pages = "899--905",

journal = "Biochemical and Biophysical Research Communications",

issn = "0006-291X",

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TY - JOUR

T1 - RNA-dependent RNA polymerase gene sequence from foot-and-mouth disease virus in Hong Kong

AU - Chen, Xi

AU - Feng, Qian

AU - Wu, Zhiyin

AU - Liu, Yingying

AU - Huang, Kun

AU - Shi, Rui

AU - Chen, Sidi

AU - Lu, Wen

AU - Ding, Mingxiao

AU - Collins, Richard A.

AU - Fung, Yin Wan Wendy

AU - Lau, Lok Ting

AU - Yu, Albert Cheung Hoi

AU - Chen, Jianguo

N1 - Funding Information: The authors thank Trevor Ellis and Pamela Li Chui Har (Agriculture, Fisheries, and Conservation Department, Veterinary Laboratory Division, Tai Lung Experimental Station, Lin Tong Mei, Sheung Shui, Hong Kong SAR, China) for providing virus samples. This work was supported by Grant G1999011904 from the Special Funds for Major State Basic Research of China to J.C. The Jun-Zheng Foundation at PKU supported X.C. Publisher copyright: © 2003 Elsevier Inc. All rights reserved.

PY - 2003/9/5

Y1 - 2003/9/5

N2 - A foot-and-mouth disease virus (FMDV, HKN/2002) was isolated in Hong Kong in 2002. The nucleotide sequence of the 3Dpol gene encoding the viral RNA-dependent RNA polymerase was determined and compared with that of the same gene from other FMDVs. The 3Dpol gene was 1410 nucleotides in length encoding a protein of 470 amino acid residues. Sequence comparisons indicated that HKN/2002 belonged to serotype O. An evolutionary tree based on the 3Dpol sequences of 20 FMDV isolates revealed that the nucleotide sequence of the HKN/2002 3Dpol gene was most similar to those of isolates found in Taiwan in 1997, suggesting that they share a common ancestor. The amino acid sequence of the HKN/2002 3Dpol gene was determined and aligned with those of representative isolates from seven other Picornaviridae genera. Eight highly conserved regions were detected, indicating a conserved functional relevance for these motifs. Alignment of 20 FMDV 3D pol amino acid sequences revealed a hypermutation region near the N-terminus that may help the virus evade host immune systems.

AB - A foot-and-mouth disease virus (FMDV, HKN/2002) was isolated in Hong Kong in 2002. The nucleotide sequence of the 3Dpol gene encoding the viral RNA-dependent RNA polymerase was determined and compared with that of the same gene from other FMDVs. The 3Dpol gene was 1410 nucleotides in length encoding a protein of 470 amino acid residues. Sequence comparisons indicated that HKN/2002 belonged to serotype O. An evolutionary tree based on the 3Dpol sequences of 20 FMDV isolates revealed that the nucleotide sequence of the HKN/2002 3Dpol gene was most similar to those of isolates found in Taiwan in 1997, suggesting that they share a common ancestor. The amino acid sequence of the HKN/2002 3Dpol gene was determined and aligned with those of representative isolates from seven other Picornaviridae genera. Eight highly conserved regions were detected, indicating a conserved functional relevance for these motifs. Alignment of 20 FMDV 3D pol amino acid sequences revealed a hypermutation region near the N-terminus that may help the virus evade host immune systems.

KW - 3D gene

KW - Foot-and-mouth disease virus

KW - Hypermutation

KW - RNA-dependent RNA polymerase

KW - Sequence alignment

KW - Serotype

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U2 - 10.1016/S0006-291X(03)01511-0

DO - 10.1016/S0006-291X(03)01511-0

M3 - Journal article

C2 - 12927804

AN - SCOPUS:0042160076

SN - 0006-291X

VL - 308

SP - 899

EP - 905

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 4

ER -

RNA-dependent RNA polymerase gene sequence from foot-and-mouth disease virus in Hong Kong

Abstract

Scopus Subject Areas

User-Defined Keywords

UN SDGs

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