Reverse-phase optode. Design and characterization of an organic acid-selective optode membrane based on fluorescein sodium

Albert W M LEE, Wing Hong CHAN*, Ke Min Wang, Zi Dong Li

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Hydrophilic gelatin membranes incorporating water soluble fluorescein sodium immobilized at the tip of an optical fiber were developed as the first 'reverse-phase' optode membrane system for the determination of organic acids as exemplified by benzoic acid. In contrast to the traditional optode membrane application, the fluorescence detection of organic acids by the present system was carried out in an organic medium (hexane). Under experimental conditions, the optical fiber coated membrane exhibited a linear response in the range 4.72 × 10-4-4.72 × 10-2 mol 1-1 benzoic acid hexane solution with a slope of 0.2 relative fluorescent intensity units per concentration decade. The selectivity, response time, reproducibility and lifetime of this optical device are discussed.

Original languageEnglish
Pages (from-to)99-105
Number of pages7
JournalAnalytica Chimica Acta
Volume322
Issue number2 JAN
DOIs
Publication statusPublished - 1996

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

User-Defined Keywords

  • Fluorimetry
  • Gelatin membrane
  • Organic acids
  • Reverse-phase optode membrane

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