Retention behaviour and fluorimetric detection of procaine hydrochloride using carboxymethyl-β-cyclodextrin as an additive in reversed-phase liquid chromatography

Shaomin Shuang, Martin M F CHOI*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

39 Citations (Scopus)

Abstract

The retention behaviour of procaine hydrochloride on an Alltima octadecyl silica (C18) column, with a mobile phase containing negatively charged carboxymethyl-β-cyclodextrin (CM-β-CD), influenced by a combination of hydrophobic and electrostatic interactions was systematically investigated. Various factors affecting the retention of procaine on the C18 column such as the concentration of CM-β-CD, pH and the methanol percentage in the mobile phase, were studied. An equation was applied to estimate the apparent binding constant of the CM-β-CD-procaine inclusion complex as an aid for understanding the retention mechanism. The first analytical application of CM-β-CD as a mobile phase additive for the determination of procaine was developed. The calibration curve was linear in the range 22-1360 ng ml-1 with an RSD of 2.1%. The detection limit based on 3σ was 1 ng ml-1 with fluorimetric detection at the excitation and emission wavelengths of 305 nm and 350 nm, respectively. The limit of quantitation based on 10σ was 22 ng ml-1. The proposed method has been successfully applied to real sample analysis.

Original languageEnglish
Pages (from-to)321-329
Number of pages9
JournalJournal of Chromatography A
Volume919
Issue number2
DOIs
Publication statusPublished - 15 Jun 2001

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

User-Defined Keywords

  • Carboxymethyl-β-cyclodextrin
  • Detection, LC
  • Fluorimetric detection
  • Procaine

Fingerprint

Dive into the research topics of 'Retention behaviour and fluorimetric detection of procaine hydrochloride using carboxymethyl-β-cyclodextrin as an additive in reversed-phase liquid chromatography'. Together they form a unique fingerprint.

Cite this