TY - JOUR
T1 - Regulatory effect of volatile compounds in fermented alcoholic beverages on gut microbiota and serum metabolism in a mouse model
AU - Ji, Mei
AU - Fang, Cheng
AU - Jia, Wei
AU - Du, Hai
AU - Xu, Yan
N1 - Funding Information:
This work was supported by the Beijing Advanced Innovation Center for Food Nutrition and Human Health, the Beijing Technology and Business University (BTBU; Grant 20182033). The authors give special thanks to the Yibin Tinghua Liquor Industry Development Co., Ltd, the Institute for Health Research of Chinese Baijiu, and the Anhui Gujing Distillery Co., Ltd. for their financial support.
Publisher Copyright:
© The Royal Society of Chemistry 2021
PY - 2021/6/21
Y1 - 2021/6/21
N2 - Ethanol (EtOH) is the main risk factor for alcoholic liver disease. However, fermented alcoholic beverages contain not only ethanol but also various volatile compounds. Currently, the effects of volatile compounds in ethanol-containing fermented beverages on gut microbiota and host metabolism are largely unclear. To fill this gap, we constructed an 8-week mice model using three types of Baijiu (a traditional fermented alcoholic beverage), sterile water and ethanol as controls. Results revealed that three types of Baijiu contain various volatiles, mainly belonging to esters, alcohols, and acids. All of Baijiu caused the lower levels of liver injury in mice than EtOH (at the same EtOH concentration) by phenotypic, biochemical and pathological analysis. We observed that Baijiu volatiles affect the gut microbiota structure and serum metabolomic patterns of mice. Compared with EtOH, Baijiu feeding profoundly increased the relative abundance of Lactobacillus (the highest relative abundance in the Baijiu-fed group was 11.16 ± 1.52%, and in the EtOH-fed group it was 1.80 ± 0.66%). Esters, acids and phenols in volatiles showed strong positive correlations (P < 0.01, R > 0.6) with Lactobacillus. Moreover, Baijiu feeding significantly (P < 0.05) enriched serum metabolites related to bioenergy (i.e., D-glucose, the highest fold change was 1.16) and anti-inflammatory activity (i.e., arachidonic acid, the highest fold change was 1.43) as compared to EtOH. Finally, the microbial and metabolic function analysis showed that volatiles will enhance the citrate cycle and biosynthesis of unsaturated fatty acid pathways. Overall, these data demonstrated the potential of volatile compounds for attenuating the progress of alcoholic liver disease by regulating the gut microbiota and host metabolism.
AB - Ethanol (EtOH) is the main risk factor for alcoholic liver disease. However, fermented alcoholic beverages contain not only ethanol but also various volatile compounds. Currently, the effects of volatile compounds in ethanol-containing fermented beverages on gut microbiota and host metabolism are largely unclear. To fill this gap, we constructed an 8-week mice model using three types of Baijiu (a traditional fermented alcoholic beverage), sterile water and ethanol as controls. Results revealed that three types of Baijiu contain various volatiles, mainly belonging to esters, alcohols, and acids. All of Baijiu caused the lower levels of liver injury in mice than EtOH (at the same EtOH concentration) by phenotypic, biochemical and pathological analysis. We observed that Baijiu volatiles affect the gut microbiota structure and serum metabolomic patterns of mice. Compared with EtOH, Baijiu feeding profoundly increased the relative abundance of Lactobacillus (the highest relative abundance in the Baijiu-fed group was 11.16 ± 1.52%, and in the EtOH-fed group it was 1.80 ± 0.66%). Esters, acids and phenols in volatiles showed strong positive correlations (P < 0.01, R > 0.6) with Lactobacillus. Moreover, Baijiu feeding significantly (P < 0.05) enriched serum metabolites related to bioenergy (i.e., D-glucose, the highest fold change was 1.16) and anti-inflammatory activity (i.e., arachidonic acid, the highest fold change was 1.43) as compared to EtOH. Finally, the microbial and metabolic function analysis showed that volatiles will enhance the citrate cycle and biosynthesis of unsaturated fatty acid pathways. Overall, these data demonstrated the potential of volatile compounds for attenuating the progress of alcoholic liver disease by regulating the gut microbiota and host metabolism.
UR - http://www.scopus.com/inward/record.url?scp=85108549000&partnerID=8YFLogxK
U2 - 10.1039/d0fo03028g
DO - 10.1039/d0fo03028g
M3 - Journal article
C2 - 34008602
AN - SCOPUS:85108549000
SN - 2042-6496
VL - 12
SP - 5576
EP - 5590
JO - Food and Function
JF - Food and Function
IS - 12
ER -