Regulatory effect of astragalus polysaccharides on intestinal intraepithelial γδT cells of tumor bearing mice

Shuyu Sun, Kang Zheng, Hongyan Zhao, Cheng Lu, Biao Liu, Changyuan Yu, Ge ZHANG, Zhaoxiang BIAN, Aiping LYU, Xiaojuan He*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

Astragalus polysaccharides (APS) possess multiple immunomodulatory activities. Due to its high molecular weight, orally administration of APS is not easily absorbed into the blood stream, and how APS exerts its capacity in vivo is still not well elucidated. We assume that enteric mucosal immune response might trigger the immune regulation of APS, and our previous studies demonstrated that APS had regulatory activity on intraepithelial lymphocytes (IELs). Therefore, this study aimed to investigate the functions of APS on intestinal intraepithelial γδT cells, a major subset in IELs and an essential component of maintaining homeostasis and immune regulation in enteric mucosa. Results showed that APS could promote proliferation and function of intestinal intraepithelial γδT cells in vitro, the IFN-γ, FasL and GrB mRNA levels in γδT cells were all significantly increased. Moreover, APS also improved the activity of intestinal intraepithelial γδT cells in vivo, as cytokines production and cytotoxicity of γδT cells were all remarkably improved in tumor-bearing mice treated with APS. In addition, the levels of TNF-α and IFN-γ were significantly increased, whereas the levels of IL-10 and TGF-β were significantly decreased in tumor-bearing mice treated with APS. In conclusion, this study demonstrated that APS could improve proliferation and function of intestinal intraepithelial γδT cells, which might an important pathway for immunomodulation of APS in cancer therapy.

Original languageEnglish
Pages (from-to)15224-15236
Number of pages13
JournalMolecules
Volume19
Issue number9
DOIs
Publication statusPublished - 23 Sep 2014

Scopus Subject Areas

  • Analytical Chemistry
  • Chemistry (miscellaneous)
  • Molecular Medicine
  • Pharmaceutical Science
  • Drug Discovery
  • Physical and Theoretical Chemistry
  • Organic Chemistry

User-Defined Keywords

  • Astragalus polysaccharides
  • Enteric mucosa
  • Tumor
  • γδT cells

Fingerprint

Dive into the research topics of 'Regulatory effect of astragalus polysaccharides on intestinal intraepithelial γδT cells of tumor bearing mice'. Together they form a unique fingerprint.

Cite this