Recombinant cysteine proteinase as anti-tick targeting Hyalomma asiaticum infestation

Ruiqi Song; Ting Ge; ercha Hu; Xinli Fan; Yang Zhang; Xuejie Zhai; Min Li; Wei Zhang; Lijiang Wu; Allen Ka Loon Cheung; Bayin Chahan

doi:10.1016/j.exppara.2022.108234

Recombinant cysteine proteinase as anti-tick targeting Hyalomma asiaticum infestation

Ruiqi Song, Ting Ge, ercha Hu, Xinli Fan, Yang Zhang, Xuejie Zhai, Min Li, Wei Zhang, Lijiang Wu, Allen Ka Loon Cheung, Bayin Chahan^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

5 Citations (Scopus)

Abstract

Cysteine proteases are involved in the digestion of host blood and the degradation of yolk proteins of arthropod ectoparasites. In this study, a cathepsin L-like cysteine proteinase gene (HasCPL) of Hyalomma asiaticum was cloned, and recombinant (r)HasCPL protein was generated for immunization study. Bioinformatic analysis confirmed HasCPL was a member of the papain family (clan CA) and have high sequence identities with CPLs of other Ixodid ticks. The efficacy of immunization against H. asiaticum infestations in rabbits was assessed. Rabbits (n = 3) were immunized three times with rHasCPL before challenged with 250 larvae per rabbit four weeks post-immunization. A high antibody titer was detected in immunized rabbits in comparison to control. Western blot analysis detected CPLs in midgut, salivary gland, and ovary. Increase of rejection percentage of larvae were noted in ticks fed on immunized animals in comparison to control. Overall, a 55.09% protection against larva ticks was noted.

Original language	English
Article number	108234
Journal	Experimental Parasitology
Volume	235
DOIs	https://doi.org/10.1016/j.exppara.2022.108234
Publication status	Published - Apr 2022

Scopus Subject Areas

Parasitology
Immunology
Infectious Diseases

User-Defined Keywords

Cysteine proteinase
Hyalomma asiaticum
Tick
Vaccine

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/j.exppara.2022.108234

Cite this

@article{a783d6c80f25485ab777ecb653550516,

title = "Recombinant cysteine proteinase as anti-tick targeting Hyalomma asiaticum infestation",

abstract = "Cysteine proteases are involved in the digestion of host blood and the degradation of yolk proteins of arthropod ectoparasites. In this study, a cathepsin L-like cysteine proteinase gene (HasCPL) of Hyalomma asiaticum was cloned, and recombinant (r)HasCPL protein was generated for immunization study. Bioinformatic analysis confirmed HasCPL was a member of the papain family (clan CA) and have high sequence identities with CPLs of other Ixodid ticks. The efficacy of immunization against H. asiaticum infestations in rabbits was assessed. Rabbits (n = 3) were immunized three times with rHasCPL before challenged with 250 larvae per rabbit four weeks post-immunization. A high antibody titer was detected in immunized rabbits in comparison to control. Western blot analysis detected CPLs in midgut, salivary gland, and ovary. Increase of rejection percentage of larvae were noted in ticks fed on immunized animals in comparison to control. Overall, a 55.09% protection against larva ticks was noted.",

keywords = "Cysteine proteinase, Hyalomma asiaticum, Tick, Vaccine",

author = "Ruiqi Song and Ting Ge and ercha Hu and Xinli Fan and Yang Zhang and Xuejie Zhai and Min Li and Wei Zhang and Lijiang Wu and Cheung, {Allen Ka Loon} and Bayin Chahan",

note = "Funding Information: This work was supported by the National Key Basic Research Program of China (2017YFD0500401). Funding Information: We thank Dr. Jia, Harbin Veterinary Research Institute, China, and Dr. Su, University of Tennessee, USA for their generous efforts in revising our manuscript. Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",

year = "2022",

month = apr,

doi = "10.1016/j.exppara.2022.108234",

language = "English",

volume = "235",

journal = "Experimental Parasitology",

issn = "0014-4894",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Recombinant cysteine proteinase as anti-tick targeting Hyalomma asiaticum infestation

AU - Song, Ruiqi

AU - Ge, Ting

AU - Hu, ercha

AU - Fan, Xinli

AU - Zhang, Yang

AU - Zhai, Xuejie

AU - Li, Min

AU - Zhang, Wei

AU - Wu, Lijiang

AU - Cheung, Allen Ka Loon

AU - Chahan, Bayin

N1 - Funding Information: This work was supported by the National Key Basic Research Program of China (2017YFD0500401). Funding Information: We thank Dr. Jia, Harbin Veterinary Research Institute, China, and Dr. Su, University of Tennessee, USA for their generous efforts in revising our manuscript. Publisher Copyright: © 2022 Elsevier Inc.

PY - 2022/4

Y1 - 2022/4

N2 - Cysteine proteases are involved in the digestion of host blood and the degradation of yolk proteins of arthropod ectoparasites. In this study, a cathepsin L-like cysteine proteinase gene (HasCPL) of Hyalomma asiaticum was cloned, and recombinant (r)HasCPL protein was generated for immunization study. Bioinformatic analysis confirmed HasCPL was a member of the papain family (clan CA) and have high sequence identities with CPLs of other Ixodid ticks. The efficacy of immunization against H. asiaticum infestations in rabbits was assessed. Rabbits (n = 3) were immunized three times with rHasCPL before challenged with 250 larvae per rabbit four weeks post-immunization. A high antibody titer was detected in immunized rabbits in comparison to control. Western blot analysis detected CPLs in midgut, salivary gland, and ovary. Increase of rejection percentage of larvae were noted in ticks fed on immunized animals in comparison to control. Overall, a 55.09% protection against larva ticks was noted.

AB - Cysteine proteases are involved in the digestion of host blood and the degradation of yolk proteins of arthropod ectoparasites. In this study, a cathepsin L-like cysteine proteinase gene (HasCPL) of Hyalomma asiaticum was cloned, and recombinant (r)HasCPL protein was generated for immunization study. Bioinformatic analysis confirmed HasCPL was a member of the papain family (clan CA) and have high sequence identities with CPLs of other Ixodid ticks. The efficacy of immunization against H. asiaticum infestations in rabbits was assessed. Rabbits (n = 3) were immunized three times with rHasCPL before challenged with 250 larvae per rabbit four weeks post-immunization. A high antibody titer was detected in immunized rabbits in comparison to control. Western blot analysis detected CPLs in midgut, salivary gland, and ovary. Increase of rejection percentage of larvae were noted in ticks fed on immunized animals in comparison to control. Overall, a 55.09% protection against larva ticks was noted.

KW - Cysteine proteinase

KW - Hyalomma asiaticum

KW - Tick

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=85125196642&partnerID=8YFLogxK

U2 - 10.1016/j.exppara.2022.108234

DO - 10.1016/j.exppara.2022.108234

M3 - Journal article

C2 - 35218759

AN - SCOPUS:85125196642

SN - 0014-4894

VL - 235

JO - Experimental Parasitology

JF - Experimental Parasitology

M1 - 108234

ER -

Recombinant cysteine proteinase as anti-tick targeting Hyalomma asiaticum infestation

Abstract

Scopus Subject Areas

User-Defined Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this