TY - JOUR
T1 - Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid
AU - Yue, Hao
AU - Chan, Wan
AU - Yu, Kongjie
AU - Guo, Lin
AU - Liu, Ning
AU - Liu, Huwei
AU - CAI, Zongwei
N1 - Funding Information:
Received August 20, 2009; accepted August 20, 2009 doi: 10.1007/s11426-009-0233-6 †Corresponding author (email: [email protected]) Supported by the Research Grant Council, University Grants Committee of Hong Kong (Grant No. HKBU2459/06M), and the Health and Health Services Research Fund of Hong Kong (Grant No. 05060141)
PY - 2009/10
Y1 - 2009/10
N2 - Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized.
AB - Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized.
KW - Aristolochic acid
KW - DNA adducts
KW - Quantitative analysis
KW - Short review
UR - http://www.scopus.com/inward/record.url?scp=70350221949&partnerID=8YFLogxK
U2 - 10.1007/s11426-009-0233-6
DO - 10.1007/s11426-009-0233-6
M3 - Journal article
AN - SCOPUS:70350221949
SN - 1006-9291
VL - 52
SP - 1576
EP - 1582
JO - Science in China, Series B: Chemistry
JF - Science in China, Series B: Chemistry
IS - 10
ER -