Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid

Hao Yue, Wan Chan, Kongjie Yu, Lin Guo, Ning Liu, Huwei Liu, Zongwei CAI*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized.

Original languageEnglish
Pages (from-to)1576-1582
Number of pages7
JournalScience in China, Series B: Chemistry
Volume52
Issue number10
DOIs
Publication statusPublished - Oct 2009

Scopus Subject Areas

  • Chemistry(all)

User-Defined Keywords

  • Aristolochic acid
  • DNA adducts
  • Quantitative analysis
  • Short review

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