Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid

Hao Yue; Wan Chan; Kongjie Yu; Lin Guo; Ning Liu; Huwei Liu; Zongwei CAI

doi:10.1007/s11426-009-0233-6

Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid

Hao Yue, Wan Chan, Kongjie Yu, Lin Guo, Ning Liu, Huwei Liu, Zongwei CAI^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized.

Original language	English
Pages (from-to)	1576-1582
Number of pages	7
Journal	Science in China, Series B: Chemistry
Volume	52
Issue number	10
DOIs	https://doi.org/10.1007/s11426-009-0233-6
Publication status	Published - Oct 2009

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Chemistry(all)

User-Defined Keywords

Aristolochic acid
DNA adducts
Quantitative analysis
Short review

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10.1007/s11426-009-0233-6

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title = "Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid",

abstract = "Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized.",

keywords = "Aristolochic acid, DNA adducts, Quantitative analysis, Short review",

author = "Hao Yue and Wan Chan and Kongjie Yu and Lin Guo and Ning Liu and Huwei Liu and Zongwei CAI",

note = "Funding Information: Received August 20, 2009; accepted August 20, 2009 doi: 10.1007/s11426-009-0233-6 †Corresponding author (email: zwcai@hkbu.edu.hk) Supported by the Research Grant Council, University Grants Committee of Hong Kong (Grant No. HKBU2459/06M), and the Health and Health Services Research Fund of Hong Kong (Grant No. 05060141)",

year = "2009",

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doi = "10.1007/s11426-009-0233-6",

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T1 - Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid

AU - Yue, Hao

AU - Chan, Wan

AU - Yu, Kongjie

AU - Guo, Lin

AU - Liu, Ning

AU - Liu, Huwei

AU - CAI, Zongwei

N1 - Funding Information: Received August 20, 2009; accepted August 20, 2009 doi: 10.1007/s11426-009-0233-6 †Corresponding author (email: zwcai@hkbu.edu.hk) Supported by the Research Grant Council, University Grants Committee of Hong Kong (Grant No. HKBU2459/06M), and the Health and Health Services Research Fund of Hong Kong (Grant No. 05060141)

PY - 2009/10

Y1 - 2009/10

N2 - Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized.

AB - Aristolochic acid (AA), a mixture of structure-related nitrophenanthrene carboxylic acid derivatives derived from Aristolochia spp, is associated with nephrotoxin and carcinogen. AA-DNA adducts induced by reductive metabolic activation of AA were detected in tissues of animals and in patients exposed to AA. The DNA adducts were generally used as biomarkers in toxicological study of AA. In this short review, quantitative analysis of AA-DNA adducts in various in vitro and in vivo systems by using 32P-postlabelling assay, HPLC-UV, HPLC-radiation monitor, HPLC-FLD, HPLC-ESI/MS and UPLC-MS/MS methods is discussed. The distribution of AA-DNA adducts in various tissues is also summarized.

KW - Aristolochic acid

KW - DNA adducts

KW - Quantitative analysis

KW - Short review

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VL - 52

SP - 1576

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JO - Science in China, Series B: Chemistry

JF - Science in China, Series B: Chemistry

IS - 10

ER -

Recent progress in quantitative analysis of DNA adducts of nephrotoxin aristolochic acid

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