Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection

Sang Cuo Nao; Ke Jia Wu; Wanhe Wang; Chung Hang Leung; Edmond Dik Lung Ma

doi:10.3389/fchem.2020.00767

Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection

Sang Cuo Nao
, Ke Jia Wu
, Wanhe Wang
, Chung Hang Leung^*
, Edmond Dik Lung Ma^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Review article › peer-review

25 Citations (Scopus)

Abstract

Ochratoxin A (OTA) is a mycotoxin that is widespread throughout the world. It contaminates foods such as vegetables, fruits, and rice. It harms human health and has potential carcinogenic effects. The G-quadruplex (G4) is a tetraplexed DNA structure generated from guanine-rich DNA that has found emerging use in aptamer-based sensing systems. This review outlines the status of OTA contamination and conventional detection methods for OTA. Various G4-based methods to detect OTA developed in recent years are summarized along with their advantages and disadvantages compared to existing approaches.

Original language	English
Article number	767
Number of pages	11
Journal	Frontiers in Chemistry
Volume	8
DOIs	https://doi.org/10.3389/fchem.2020.00767
Publication status	Published - 1 Sept 2020

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

aptamer
food safety
G-quadruplex
nucleic acid detection
ochratoxin A

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10.3389/fchem.2020.00767

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title = "Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection",

abstract = "Ochratoxin A (OTA) is a mycotoxin that is widespread throughout the world. It contaminates foods such as vegetables, fruits, and rice. It harms human health and has potential carcinogenic effects. The G-quadruplex (G4) is a tetraplexed DNA structure generated from guanine-rich DNA that has found emerging use in aptamer-based sensing systems. This review outlines the status of OTA contamination and conventional detection methods for OTA. Various G4-based methods to detect OTA developed in recent years are summarized along with their advantages and disadvantages compared to existing approaches.",

keywords = "aptamer, food safety, G-quadruplex, nucleic acid detection, ochratoxin A",

author = "Nao, \{Sang Cuo\} and Wu, \{Ke Jia\} and Wanhe Wang and Leung, \{Chung Hang\} and Ma, \{Edmond Dik Lung\}",

note = "Funding Information: Funding. This work was supported by Hong Kong Baptist University (FRG2/17-18/003), the Health and Medical Research Fund (HMRF/14150561), the National Natural Science Foundation of China (21575121 and 21775131), the Hong Kong Baptist University Century Club Sponsorship Scheme 2019 and 2020, the Interdisciplinary Research Matching Scheme (RC-IRMS/16- 17/03), Interdisciplinary Research Clusters Matching Scheme (RC-IRCs/17-18/03), Collaborative Research Fund (C5026- 16G), SKLEBA and HKBU Strategic Development Fund (SKLP\_1920\_P02), the Science and Technology Development Fund, Macau SAR (File no. 0072/2018/A2), and the University of Macau (MYRG2018-00187-ICMS and MYRG-2019- 00002-ICMS).",

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doi = "10.3389/fchem.2020.00767",

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AU - Nao, Sang Cuo

AU - Wu, Ke Jia

AU - Wang, Wanhe

AU - Leung, Chung Hang

AU - Ma, Edmond Dik Lung

N1 - Funding Information: Funding. This work was supported by Hong Kong Baptist University (FRG2/17-18/003), the Health and Medical Research Fund (HMRF/14150561), the National Natural Science Foundation of China (21575121 and 21775131), the Hong Kong Baptist University Century Club Sponsorship Scheme 2019 and 2020, the Interdisciplinary Research Matching Scheme (RC-IRMS/16- 17/03), Interdisciplinary Research Clusters Matching Scheme (RC-IRCs/17-18/03), Collaborative Research Fund (C5026- 16G), SKLEBA and HKBU Strategic Development Fund (SKLP_1920_P02), the Science and Technology Development Fund, Macau SAR (File no. 0072/2018/A2), and the University of Macau (MYRG2018-00187-ICMS and MYRG-2019- 00002-ICMS).

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N2 - Ochratoxin A (OTA) is a mycotoxin that is widespread throughout the world. It contaminates foods such as vegetables, fruits, and rice. It harms human health and has potential carcinogenic effects. The G-quadruplex (G4) is a tetraplexed DNA structure generated from guanine-rich DNA that has found emerging use in aptamer-based sensing systems. This review outlines the status of OTA contamination and conventional detection methods for OTA. Various G4-based methods to detect OTA developed in recent years are summarized along with their advantages and disadvantages compared to existing approaches.

AB - Ochratoxin A (OTA) is a mycotoxin that is widespread throughout the world. It contaminates foods such as vegetables, fruits, and rice. It harms human health and has potential carcinogenic effects. The G-quadruplex (G4) is a tetraplexed DNA structure generated from guanine-rich DNA that has found emerging use in aptamer-based sensing systems. This review outlines the status of OTA contamination and conventional detection methods for OTA. Various G4-based methods to detect OTA developed in recent years are summarized along with their advantages and disadvantages compared to existing approaches.

KW - aptamer

KW - food safety

KW - G-quadruplex

KW - nucleic acid detection

KW - ochratoxin A

UR - https://www.scopus.com/pages/publications/85090933145

U2 - 10.3389/fchem.2020.00767

DO - 10.3389/fchem.2020.00767

M3 - Review article

AN - SCOPUS:85090933145

SN - 2296-2646

VL - 8

JO - Frontiers in Chemistry

JF - Frontiers in Chemistry

M1 - 767

ER -

Recent Progress and Development of G-Quadruplex-Based Luminescent Assays for Ochratoxin A Detection

Abstract

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