TY - JOUR
T1 - Recent methods for purification and structure determination of oligonucleotides
AU - Zhang, Qiulong
AU - Lv, Huanhuan
AU - Wang, Lili
AU - Chen, Man
AU - Li, Fangfei
AU - Liang, Chao
AU - Yu, Yuanyuan
AU - Jiang, Feng
AU - Lyu, Aiping
AU - Zhang, Ge
N1 - Funding Information:
We thank academic staff members in Aiping Lu and Ge Zhang?s group at Hong Kong Baptist University. We also thank Hong Kong Baptist University for providing critical comments and technical support. This study was supported by Hong Kong General Research Fund (HKBU262913 to Ge Zhang), Science and Technology Innovation Commission of Shenzhen Municipality (SCM-2016-SZTIC-001 to Ge Zhang) and National Natural Science Foundation of China (NSFC 81673773 to Aiping Lu).
PY - 2016/12/18
Y1 - 2016/12/18
N2 - Aptamers are single-stranded DNA or RNA oligonucleotides that can interact with target molecules through specific three-dimensional structures. The excellent features, such as high specificity and affinity for target proteins, small size, chemical stability, low immunogenicity, facile chemical synthesis, versatility in structural design and engineering, and accessible for site-specific modifications with functional moieties, make aptamers attractive molecules in the fields of clinical diagnostics and biopharmaceutical therapeutics. However, difficulties in purification and structural identification of aptamers remain a major impediment to their broad clinical application. In this mini-review, we present the recently attractive developments regarding the purification and identification of aptamers. We also discuss the advantages, limitations, and prospects for the major methods applied in purifying and identifying aptamers, which could facilitate the application of aptamers.
AB - Aptamers are single-stranded DNA or RNA oligonucleotides that can interact with target molecules through specific three-dimensional structures. The excellent features, such as high specificity and affinity for target proteins, small size, chemical stability, low immunogenicity, facile chemical synthesis, versatility in structural design and engineering, and accessible for site-specific modifications with functional moieties, make aptamers attractive molecules in the fields of clinical diagnostics and biopharmaceutical therapeutics. However, difficulties in purification and structural identification of aptamers remain a major impediment to their broad clinical application. In this mini-review, we present the recently attractive developments regarding the purification and identification of aptamers. We also discuss the advantages, limitations, and prospects for the major methods applied in purifying and identifying aptamers, which could facilitate the application of aptamers.
KW - Aptamers
KW - Identification
KW - Purification
UR - http://www.scopus.com/inward/record.url?scp=85007028729&partnerID=8YFLogxK
U2 - 10.3390/ijms17122134
DO - 10.3390/ijms17122134
M3 - Journal article
C2 - 27999357
AN - SCOPUS:85007028729
SN - 1661-6596
VL - 17
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 12
M1 - 2134
ER -