Recent development of g-quadruplex probes for cellular imaging

Edmond Dik Lung MA*, Modi Wang, Sheng Lin, Simon Q B HAN, Chung Hang Leung

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

The G-quadruplex structure is a non-canonical secondary DNA motif that is built from planar tetrads of guanine residues stabilized by Hoogsteen-type hydrogen bonding. Bioinformatics studies indicate that sequences rich in guanine that are able to form G-quadruplexes are widely distributed throughout the human genome, particularly in telomere and promoter regions of genes. G-quadruplex sequences found in the promoters of human oncogenes, such as k-ras, c-myc and bcl-2 have attracted particular attention as molecular targets for therapeutic intervention due to their potential capability to regulate gene expression at the transcriptional level. Moreover, the G-quadruplex structure has been associated with a number of human diseases arising from defective telomeric maintenance. Despite intensive research in this area, however, the actual function of G-quadruplexes in vivo has not yet been fully understood. Therefore, significant efforts have been devoted to the discovery of specific probes for visualizing and distinguishing G-quadruplex structures from other nucleic acid molecules likely to be found in biological environments. This review summarizes recent studies in the development of G-quadruplex probes over the past three years, with a particular emphasize on the detection and imaging of G-quadruplex structures within living cells. Furthermore, the detection and biological relevance of RNA G-quadruplexes is discussed.

Original languageEnglish
Pages (from-to)1957-1963
Number of pages7
JournalCurrent Topics in Medicinal Chemistry
Volume15
Issue number19
DOIs
Publication statusPublished - 1 Jul 2015

Scopus Subject Areas

  • Drug Discovery

User-Defined Keywords

  • Cell imaging
  • DNA
  • G-quadruplex
  • Probe

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