TY - JOUR
T1 - Recent advances in SELEX technology and aptamer applications in biomedicine
AU - Zhuo, Zhenjian
AU - YU, Yuanyuan
AU - Wang, Maolin
AU - Li, Jie
AU - Zhang, Zongkang
AU - LIU, Jin
AU - Wu, Xiaohao
AU - LYU, Aiping
AU - ZHANG, Ge
AU - Zhang, Baoting
N1 - Funding Information:
Acknowledgments: This study was supported by the Hong Kong General Research Fund (14112915) and the direct grant of The Chinese University of Hong Kong (4054220 and 4054138).
Funding Information:
School of Chinese Medicine, The Chinese University of Hong Kong, Hong Kong 999077, China; [email protected] (Z.Z.); [email protected] (J.L.); [email protected] (Z.Z.) Law Sau Fai Institute for Advancing Translational Medicine in Bone & Joint Diseases, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China; [email protected] (Y.Y.); [email protected] (M.W.); [email protected] (J.L.); [email protected] (X.W.) Institute of Integrated Bioinfomedicine and Translational Science, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China Institute of Precision Medicine and Innovative Drug Discovery, School of Chinese Medicine, Hong Kong Baptist University, Hong Kong 999077, China Shenzhen Lab of Combinatorial Compounds and Targeted Drug Delivery, HKBU Institute of Research and Continuing Education, Shenzhen 518000, China Correspondence: [email protected] (A.L.); [email protected] (G.Z.); [email protected] (B.Z.); Tel.: +852-3411-2456 (A.L.); +852-3411-2958 (G.Z.); +852-39434285 (B.Z.) These authors contributed equally to this work.
PY - 2017/10/14
Y1 - 2017/10/14
N2 - Aptamers are short DNA/RNA oligonucleotides capable of binding to target molecules with high affinity and specificity. The process of selecting an aptamer is called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Thanks to the inherit merits, aptamers have been used in a wide range of applications, including disease diagnosis, targeted delivery agents and therapeutic uses. To date, great achievements regarding the selection, modifications and application of aptamers have been made. However, few aptamer-based products have already successfully entered into clinical and industrial use. Besides, it is still a challenge to obtain aptamers with high affinity in a more efficient way. Thus, it is important to comprehensively review the current shortage and achievement of aptamer-related technology. In this review, we first present the limitations and notable advances of aptamer selection. Then, we compare the different methods used in the kinetic characterization of aptamers. We also discuss the impetus and developments of the clinical application of aptamers.
AB - Aptamers are short DNA/RNA oligonucleotides capable of binding to target molecules with high affinity and specificity. The process of selecting an aptamer is called Systematic Evolution of Ligands by Exponential Enrichment (SELEX). Thanks to the inherit merits, aptamers have been used in a wide range of applications, including disease diagnosis, targeted delivery agents and therapeutic uses. To date, great achievements regarding the selection, modifications and application of aptamers have been made. However, few aptamer-based products have already successfully entered into clinical and industrial use. Besides, it is still a challenge to obtain aptamers with high affinity in a more efficient way. Thus, it is important to comprehensively review the current shortage and achievement of aptamer-related technology. In this review, we first present the limitations and notable advances of aptamer selection. Then, we compare the different methods used in the kinetic characterization of aptamers. We also discuss the impetus and developments of the clinical application of aptamers.
KW - Aptamer
KW - Selection
KW - SELEX
KW - Targeted drug delivery systems
KW - Therapy
UR - http://www.scopus.com/inward/record.url?scp=85032016808&partnerID=8YFLogxK
U2 - 10.3390/ijms18102142
DO - 10.3390/ijms18102142
M3 - Review article
C2 - 29036890
AN - SCOPUS:85032016808
SN - 1661-6596
VL - 18
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
IS - 10
M1 - 2142
ER -