Ratiometric fluorescent detection of pesticide based on split aptamer and magnetic separation

Ying Wang; Fuyan Zhu; Li Yin; Guangxu Qu; Dik Lung Ma; Chung Hang Leung; Lihua Lu

doi:10.1016/j.snb.2022.132045

Ratiometric fluorescent detection of pesticide based on split aptamer and magnetic separation

Ying Wang, Fuyan Zhu, Li Yin, Guangxu Qu, Dik Lung Ma^*, Chung Hang Leung^*, Lihua Lu^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

14 Citations (Scopus)

Abstract

Pesticide residues have seriously threatened human health and affected the sustainable development of the environment. It is pivotal to establish an efficient detection platform for pesticide assays. A novel ratiometric fluorescent aptasensor for sensitive determination of pesticide has been successfully established. Carbon dots (CDs) are used as the reference emitter, while the T-rich single-stranded DNA templated copper nanoparticles (CuNPs) provide the increasing signal. More precisely, CDs/magnetic beads (MBs) combined with split aptamer are used in this detection platform to present a label-free and enzyme-free isocarbophos (ICP) detection with high sensitivity and specificity. In the presence of ICP, a sandwich-typed complex is formed which attaches a T-rich DNA. After magnetic separation and addition of Cu²⁺/ascorbic acid (AA), the CuNPs are generated and the strong red luminescent appears. Without ICP, just blue emission from CDs exits. The developed ratiometric aptasensor offers an excellent ICP detection in the linear range of 3–200 nM with a detection limit of 0.81 nM. It provides new ways for the assay of pesticides in agricultural products or environmental samples.

Original language	English
Article number	132045
Journal	Sensors and Actuators B: Chemical
Volume	367
DOIs	https://doi.org/10.1016/j.snb.2022.132045
Publication status	Published - 15 Sept 2022

Scopus Subject Areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

User-Defined Keywords

Aptasensor
Carbon dot
CuNPs
Pesticide
Ratiometric
Split aptamer

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.snb.2022.132045

Cite this

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title = "Ratiometric fluorescent detection of pesticide based on split aptamer and magnetic separation",

abstract = "Pesticide residues have seriously threatened human health and affected the sustainable development of the environment. It is pivotal to establish an efficient detection platform for pesticide assays. A novel ratiometric fluorescent aptasensor for sensitive determination of pesticide has been successfully established. Carbon dots (CDs) are used as the reference emitter, while the T-rich single-stranded DNA templated copper nanoparticles (CuNPs) provide the increasing signal. More precisely, CDs/magnetic beads (MBs) combined with split aptamer are used in this detection platform to present a label-free and enzyme-free isocarbophos (ICP) detection with high sensitivity and specificity. In the presence of ICP, a sandwich-typed complex is formed which attaches a T-rich DNA. After magnetic separation and addition of Cu2+/ascorbic acid (AA), the CuNPs are generated and the strong red luminescent appears. Without ICP, just blue emission from CDs exits. The developed ratiometric aptasensor offers an excellent ICP detection in the linear range of 3–200 nM with a detection limit of 0.81 nM. It provides new ways for the assay of pesticides in agricultural products or environmental samples.",

keywords = "Aptasensor, Carbon dot, CuNPs, Pesticide, Ratiometric, Split aptamer",

author = "Ying Wang and Fuyan Zhu and Li Yin and Guangxu Qu and Ma, {Dik Lung} and Leung, {Chung Hang} and Lihua Lu",

note = "Funding Information: This work was funded by the National Natural Science Foundation of China (nos. 21705089 , 22077109 ), the Natural Science Foundation of Shandong Province (no. ZR2017MB064 ), Science and Technology Program of Qingdao (no. 18–6-1–83-nsh ), and the Project of Shandong Province Higher Educational Science and Technology Program (no. J17KA109 ). Publisher Copyright: {\textcopyright} 2022 Elsevier B.V.",

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doi = "10.1016/j.snb.2022.132045",

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T1 - Ratiometric fluorescent detection of pesticide based on split aptamer and magnetic separation

AU - Wang, Ying

AU - Zhu, Fuyan

AU - Yin, Li

AU - Qu, Guangxu

AU - Ma, Dik Lung

AU - Leung, Chung Hang

AU - Lu, Lihua

N1 - Funding Information: This work was funded by the National Natural Science Foundation of China (nos. 21705089 , 22077109 ), the Natural Science Foundation of Shandong Province (no. ZR2017MB064 ), Science and Technology Program of Qingdao (no. 18–6-1–83-nsh ), and the Project of Shandong Province Higher Educational Science and Technology Program (no. J17KA109 ). Publisher Copyright: © 2022 Elsevier B.V.

PY - 2022/9/15

Y1 - 2022/9/15

N2 - Pesticide residues have seriously threatened human health and affected the sustainable development of the environment. It is pivotal to establish an efficient detection platform for pesticide assays. A novel ratiometric fluorescent aptasensor for sensitive determination of pesticide has been successfully established. Carbon dots (CDs) are used as the reference emitter, while the T-rich single-stranded DNA templated copper nanoparticles (CuNPs) provide the increasing signal. More precisely, CDs/magnetic beads (MBs) combined with split aptamer are used in this detection platform to present a label-free and enzyme-free isocarbophos (ICP) detection with high sensitivity and specificity. In the presence of ICP, a sandwich-typed complex is formed which attaches a T-rich DNA. After magnetic separation and addition of Cu2+/ascorbic acid (AA), the CuNPs are generated and the strong red luminescent appears. Without ICP, just blue emission from CDs exits. The developed ratiometric aptasensor offers an excellent ICP detection in the linear range of 3–200 nM with a detection limit of 0.81 nM. It provides new ways for the assay of pesticides in agricultural products or environmental samples.

AB - Pesticide residues have seriously threatened human health and affected the sustainable development of the environment. It is pivotal to establish an efficient detection platform for pesticide assays. A novel ratiometric fluorescent aptasensor for sensitive determination of pesticide has been successfully established. Carbon dots (CDs) are used as the reference emitter, while the T-rich single-stranded DNA templated copper nanoparticles (CuNPs) provide the increasing signal. More precisely, CDs/magnetic beads (MBs) combined with split aptamer are used in this detection platform to present a label-free and enzyme-free isocarbophos (ICP) detection with high sensitivity and specificity. In the presence of ICP, a sandwich-typed complex is formed which attaches a T-rich DNA. After magnetic separation and addition of Cu2+/ascorbic acid (AA), the CuNPs are generated and the strong red luminescent appears. Without ICP, just blue emission from CDs exits. The developed ratiometric aptasensor offers an excellent ICP detection in the linear range of 3–200 nM with a detection limit of 0.81 nM. It provides new ways for the assay of pesticides in agricultural products or environmental samples.

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KW - Carbon dot

KW - CuNPs

KW - Pesticide

KW - Ratiometric

KW - Split aptamer

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ER -

Ratiometric fluorescent detection of pesticide based on split aptamer and magnetic separation

Abstract

Scopus Subject Areas

User-Defined Keywords

UN SDGs

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