Ratiometric emission fluorescent pH probe for imaging of living cells in extreme acidity

Weifen Niu; Li Fan; Ming Nan; Zengbo Li; Dongtao Lu; Man Shing Wong; Shaomin Shuang; Chuan Dong

doi:10.1021/ac504109h

Ratiometric emission fluorescent pH probe for imaging of living cells in extreme acidity

Weifen Niu, Li Fan, Ming Nan, Zengbo Li, Dongtao Lu, Man Shing Wong, Shaomin Shuang, Chuan Dong^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

115 Citations (Scopus)

Abstract

A novel ratiometric emission fluorescent probe, 1,1-dimethyl-2-[2-(quinolin-4-yl)vinyl]-1H-benzo[e]indole (QVBI), is facilely synthesized via ethylene bridging of benzoindole and quinoline. The probe exhibits ratiometric fluorescence emission (F_522nm/F_630nm) characteristics with pK_a 3.27 and linear response to extreme-acidity range of 3.8-2.0. Also, its high fluorescence quantum yield (φ = 0.89) and large Stokes shift (110 nm) are favorable. Moreover, QVBI possesses highly selective response to H⁺ over metal ions and some bioactive molecules, good photostability, and excellent reversibility. The probe has excellent cell membrane permeability and is further applied successfully to monitor pH fluctuations in live cells and imaging extreme acidity in Escherichia coli cells without influence of autofluorescence and native cellular species in biological systems.

Original language	English
Pages (from-to)	2788-2793
Number of pages	6
Journal	Analytical Chemistry
Volume	87
Issue number	5
DOIs	https://doi.org/10.1021/ac504109h
Publication status	Published - 3 Mar 2015

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Analytical Chemistry

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@article{d7d6e46118fe4745ab26e91b4116cc12,

title = "Ratiometric emission fluorescent pH probe for imaging of living cells in extreme acidity",

abstract = "A novel ratiometric emission fluorescent probe, 1,1-dimethyl-2-[2-(quinolin-4-yl)vinyl]-1H-benzo[e]indole (QVBI), is facilely synthesized via ethylene bridging of benzoindole and quinoline. The probe exhibits ratiometric fluorescence emission (F522nm/F630nm) characteristics with pKa 3.27 and linear response to extreme-acidity range of 3.8-2.0. Also, its high fluorescence quantum yield (φ = 0.89) and large Stokes shift (110 nm) are favorable. Moreover, QVBI possesses highly selective response to H+ over metal ions and some bioactive molecules, good photostability, and excellent reversibility. The probe has excellent cell membrane permeability and is further applied successfully to monitor pH fluctuations in live cells and imaging extreme acidity in Escherichia coli cells without influence of autofluorescence and native cellular species in biological systems.",

author = "Weifen Niu and Li Fan and Ming Nan and Zengbo Li and Dongtao Lu and Wong, {Man Shing} and Shaomin Shuang and Chuan Dong",

note = "Funding information: This work was supported by the National Natural Science Foundation of China (21175086, 21175087, and 21475080), the Shanxi International S&T Cooperation Program of China (2011081017), and Natural Science Foundation of Chongqing Municipal Education Commission (KJ1401210). We appreciate Shanxi Medical University (Taiyuan, China) for kindly providing us with the living cells. M.S.W. thanks the Hundred Talent Program of Shanxi Province for the financial support. Publisher copyright: {\textcopyright} 2015 American Chemical Society",

year = "2015",

month = mar,

day = "3",

doi = "10.1021/ac504109h",

language = "English",

volume = "87",

pages = "2788--2793",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

number = "5",

}

TY - JOUR

T1 - Ratiometric emission fluorescent pH probe for imaging of living cells in extreme acidity

AU - Niu, Weifen

AU - Fan, Li

AU - Nan, Ming

AU - Li, Zengbo

AU - Lu, Dongtao

AU - Wong, Man Shing

AU - Shuang, Shaomin

AU - Dong, Chuan

N1 - Funding information: This work was supported by the National Natural Science Foundation of China (21175086, 21175087, and 21475080), the Shanxi International S&T Cooperation Program of China (2011081017), and Natural Science Foundation of Chongqing Municipal Education Commission (KJ1401210). We appreciate Shanxi Medical University (Taiyuan, China) for kindly providing us with the living cells. M.S.W. thanks the Hundred Talent Program of Shanxi Province for the financial support. Publisher copyright: © 2015 American Chemical Society

PY - 2015/3/3

Y1 - 2015/3/3

N2 - A novel ratiometric emission fluorescent probe, 1,1-dimethyl-2-[2-(quinolin-4-yl)vinyl]-1H-benzo[e]indole (QVBI), is facilely synthesized via ethylene bridging of benzoindole and quinoline. The probe exhibits ratiometric fluorescence emission (F522nm/F630nm) characteristics with pKa 3.27 and linear response to extreme-acidity range of 3.8-2.0. Also, its high fluorescence quantum yield (φ = 0.89) and large Stokes shift (110 nm) are favorable. Moreover, QVBI possesses highly selective response to H+ over metal ions and some bioactive molecules, good photostability, and excellent reversibility. The probe has excellent cell membrane permeability and is further applied successfully to monitor pH fluctuations in live cells and imaging extreme acidity in Escherichia coli cells without influence of autofluorescence and native cellular species in biological systems.

AB - A novel ratiometric emission fluorescent probe, 1,1-dimethyl-2-[2-(quinolin-4-yl)vinyl]-1H-benzo[e]indole (QVBI), is facilely synthesized via ethylene bridging of benzoindole and quinoline. The probe exhibits ratiometric fluorescence emission (F522nm/F630nm) characteristics with pKa 3.27 and linear response to extreme-acidity range of 3.8-2.0. Also, its high fluorescence quantum yield (φ = 0.89) and large Stokes shift (110 nm) are favorable. Moreover, QVBI possesses highly selective response to H+ over metal ions and some bioactive molecules, good photostability, and excellent reversibility. The probe has excellent cell membrane permeability and is further applied successfully to monitor pH fluctuations in live cells and imaging extreme acidity in Escherichia coli cells without influence of autofluorescence and native cellular species in biological systems.

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U2 - 10.1021/ac504109h

DO - 10.1021/ac504109h

M3 - Journal article

C2 - 25664606

AN - SCOPUS:84923884585

SN - 0003-2700

VL - 87

SP - 2788

EP - 2793

JO - Analytical Chemistry

JF - Analytical Chemistry

IS - 5

ER -

Ratiometric emission fluorescent pH probe for imaging of living cells in extreme acidity

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