TY - JOUR
T1 - Rapid screening of 70 colorants in dyeable foods by using ultra-high-performance liquid chromatography–hybrid quadrupole–Orbitrap mass spectrometry with customized accurate-mass database and mass spectral library
AU - Wu, Wenlin
AU - Liu, Shiyao
AU - Guo, Tianrong
AU - Han, Xiying
AU - Xia, Bing
AU - Wan, Yuping
AU - Han, Quanbin
AU - Zhou, Yan
N1 - Funding Information:
Dr. Yan Zhou received funding from the National Natural Science Foundation of China (No. 21772193) and the National Key Research and Development Program of China (No. 2018YFC1602400).
Dr. Bing Xia received funding from the National Natural Science Foundation of China (No. 21672206).
Dr. Quanbin Han received funding from the Science and Technology Project of Shenzhen (No. JCYJ20160531193812867).
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/9/15
Y1 - 2021/9/15
N2 - A rapid screening method of 70 colorants for regulatory control in dyeable foods was established using ultra-high-performance liquid chromatography–hybrid quadrupole–Orbitrap mass spectrometry (UHPLC-Q/Orbitrap MS) with customized accurate-mass database and mass spectral library. A rapid, high-throughput, and simple sample pretreatment condition with low reagent consumption and high recovery was developed on the basis of ultrasound-assisted extraction and dispersion solid-phase extraction. Rapid screening was conducted by comparing the experimentally measured exact mass of the parent and fragment ions, the isotope pattern, and the retention time with the accurate-mass database and by matching the acquired MS/MS spectra against the mass spectral library. The performance of the method was evaluated in terms of linearity, limits of detection, limits of quantitation, recovery, repeatability, reproducibility, and matrix effect. The proposed method was applied for simultaneous analysis of 70 colorants in seven kinds of dyeable foods, and it exhibited great potential for broad, sensitive, and reliable.
AB - A rapid screening method of 70 colorants for regulatory control in dyeable foods was established using ultra-high-performance liquid chromatography–hybrid quadrupole–Orbitrap mass spectrometry (UHPLC-Q/Orbitrap MS) with customized accurate-mass database and mass spectral library. A rapid, high-throughput, and simple sample pretreatment condition with low reagent consumption and high recovery was developed on the basis of ultrasound-assisted extraction and dispersion solid-phase extraction. Rapid screening was conducted by comparing the experimentally measured exact mass of the parent and fragment ions, the isotope pattern, and the retention time with the accurate-mass database and by matching the acquired MS/MS spectra against the mass spectral library. The performance of the method was evaluated in terms of linearity, limits of detection, limits of quantitation, recovery, repeatability, reproducibility, and matrix effect. The proposed method was applied for simultaneous analysis of 70 colorants in seven kinds of dyeable foods, and it exhibited great potential for broad, sensitive, and reliable.
KW - Accurate-mass database
KW - Colorants
KW - Dyeable foods
KW - Hybrid quadrupole–Orbitrap mass spectrometry
KW - Mass spectral library
KW - Ultra-high-performance liquid chromatography
UR - http://www.scopus.com/inward/record.url?scp=85105285868&partnerID=8YFLogxK
U2 - 10.1016/j.foodchem.2021.129643
DO - 10.1016/j.foodchem.2021.129643
M3 - Journal article
C2 - 33812190
AN - SCOPUS:85105285868
SN - 0308-8146
VL - 356
JO - Food Chemistry
JF - Food Chemistry
M1 - 129643
ER -