Rapid identification of direct-acting pancreatic protectants from Cyclocarya paliurus leaves tea by the method of serum pharmacochemistry combined with target cell extraction

Wei Hong Chen, Zhen Luo, Zi Wan Ning, Jiao Peng, Xiao Peng Hu, Li Xiang Zhai, Bo Wen, Haitao XIAO*, Zhaoxiang BIAN

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Extracts of Cyclocarya paliurus (CP) leaves, a popular sweet tea, inhibit pancreatic β cell apoptosis and have potent hypoglycemic effects, but the identities of the anti-apoptotic bioactive components are still unknown. In the present study, a method using UPLC-Q-TOF/MS based on serum pharmacochemistry combined with target cell extraction was established to rapidly identify direct-acting pancreatic protectants from CP. After orally administering a set amount of CP extract to rats, blood samples were collected to characterize the components that can be absorbed into the blood using UPLC-Q-TOF/MS. Also, target cells (pancreatic β NIT-1 cells) were incubated with CP extract for 24 hours, and cells were collected to identify the components that can bind to the cells using UPLC-Q-TOF/MS. Finally, to evaluate the protective effect of the bioactive components of CP, MTT and TUNEL assays were performed on treated NIT-1 cell induced by streptozotocin (STZ). Three potential direct-acting pancreatic protectants-kaempferol, quercetin, quadranoside IV-were identified, and anti-apoptotic effects of kaempferol and quercetin were confirmed in STZ-induced NIT-1 cells. The findings indicate that this combined approach is a feasible, rapid, and expedient tool for capturing potential direct-acting components from natural products such as those from CP leaves.

Original languageEnglish
Pages (from-to)265-274
Number of pages10
JournalOpen Chemistry
Volume18
Issue number1
DOIs
Publication statusPublished - 1 Jan 2020

Scopus Subject Areas

  • Chemistry(all)
  • Materials Chemistry

User-Defined Keywords

  • Cyclocarya paliurus
  • pancreatic protectants
  • serum pharmacochemistry
  • target cell extraction
  • UPLC-Q-TOF/MS

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