TY - JOUR
T1 - Rapid determination of polyprenylated xanthones in gamboge resin of Garcinia hanburyi by HPLC
AU - Song, Jing Zheng
AU - Yip, Yue Keung
AU - Han, Quan Bin
AU - Qiao, Chun Feng
AU - Xu, Hong Xi
N1 - Research Funding: Hong Kong Jockey Club Charities Trust
PY - 2007/2/13
Y1 - 2007/2/13
N2 - A rapid ion-pair HPLC method was developed and validated for the determination of eight polyprenylated xanthones including three pairs of epimers, namely morellic acid (MA), 30-hydroxygambogic acid (HGA), 30-hydroxyepigambogic acid (HEGA), isogambogic acid (IGA), epiisogambogic acid (EIGA), gambogenic acid (GNA), gambogic acid (GA), and epigambogic acid (EGA), in gamboge resin of Garcinia hanburyi. The separation was performed on a narrow bore C8 column with isocratic elution using a mixture of methanol-ACN-40 mM KH2PO4 buffer (37.5:37.5:25 v/v/v, containing 0.1% tetradecyltrimethylammonium bromide). The newly developed method was used to determine the contents of the eight compounds present in the gamboge. Results showed that GA and EGA are the dominant components of gamboge. The content ratio of each epimer pair remained constant, indicating that the content ratio of epimers can be used as a specific characteristic for the quality control of gamboge.
AB - A rapid ion-pair HPLC method was developed and validated for the determination of eight polyprenylated xanthones including three pairs of epimers, namely morellic acid (MA), 30-hydroxygambogic acid (HGA), 30-hydroxyepigambogic acid (HEGA), isogambogic acid (IGA), epiisogambogic acid (EIGA), gambogenic acid (GNA), gambogic acid (GA), and epigambogic acid (EGA), in gamboge resin of Garcinia hanburyi. The separation was performed on a narrow bore C8 column with isocratic elution using a mixture of methanol-ACN-40 mM KH2PO4 buffer (37.5:37.5:25 v/v/v, containing 0.1% tetradecyltrimethylammonium bromide). The newly developed method was used to determine the contents of the eight compounds present in the gamboge. Results showed that GA and EGA are the dominant components of gamboge. The content ratio of each epimer pair remained constant, indicating that the content ratio of epimers can be used as a specific characteristic for the quality control of gamboge.
KW - Epimers
KW - Gamboge
KW - Gambogic acid
KW - High performance liquid chromatography (HPLC)
KW - Polyprenylated xanthone
UR - http://www.scopus.com/inward/record.url?scp=33847655496&partnerID=8YFLogxK
UR - https://pubmed.ncbi.nlm.nih.gov/17396587/#affiliation-1
U2 - 10.1002/jssc.200600294
DO - 10.1002/jssc.200600294
M3 - Journal article
AN - SCOPUS:33847655496
SN - 1615-9306
VL - 30
SP - 304
EP - 309
JO - Journal of Separation Science
JF - Journal of Separation Science
IS - 3
ER -