Rapid determination of polyprenylated xanthones in gamboge resin of Garcinia hanburyi by HPLC

Jing Zheng Song, Yue Keung Yip, Quan Bin Han, Chun Feng Qiao, Hong Xi Xu*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

34 Citations (Scopus)


A rapid ion-pair HPLC method was developed and validated for the determination of eight polyprenylated xanthones including three pairs of epimers, namely morellic acid (MA), 30-hydroxygambogic acid (HGA), 30-hydroxyepigambogic acid (HEGA), isogambogic acid (IGA), epiisogambogic acid (EIGA), gambogenic acid (GNA), gambogic acid (GA), and epigambogic acid (EGA), in gamboge resin of Garcinia hanburyi. The separation was performed on a narrow bore C8 column with isocratic elution using a mixture of methanol-ACN-40 mM KH2PO4 buffer (37.5:37.5:25 v/v/v, containing 0.1% tetradecyltrimethylammonium bromide). The newly developed method was used to determine the contents of the eight compounds present in the gamboge. Results showed that GA and EGA are the dominant components of gamboge. The content ratio of each epimer pair remained constant, indicating that the content ratio of epimers can be used as a specific characteristic for the quality control of gamboge.

Original languageEnglish
Pages (from-to)304-309
Number of pages6
JournalJournal of Separation Science
Issue number3
Publication statusPublished - 13 Feb 2007

Scopus Subject Areas

  • Analytical Chemistry
  • Filtration and Separation

User-Defined Keywords

  • Epimers
  • Gamboge
  • Gambogic acid
  • High performance liquid chromatography (HPLC)
  • Polyprenylated xanthone


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