TY - JOUR
T1 - Rapid biodegradation of benzo[a]pyrene by bacillus subtilis BUM under thermophilic condition
AU - Zhao, Zhenyong
AU - Wong, Jonathan Woon Chung
N1 - Copyright:
Copyright 2011 Elsevier B.V., All rights reserved.
PY - 2010/11/1
Y1 - 2010/11/1
N2 - Two thermophilic bacterial strains, Bacillus subtilis BUM (BUM) and Mycobacterium vanbaalenii BU42 (BU42), were tested for their potential in biodegradation of benzo[a]pyrene. Neither BUM nor BU42 utilized benzo[a]pyrene as the sole substrate under thermophilic condition. In the presence of 50, 250, and 500 mg L-1 phenanthrene, the biodegradation of benzo[a]pyrene by BUM occurred and removals in 30 days were 14.8%, 38.8%, and 63.6%, respectively. This is the first report on the biodegradation of benzo[a]pyrene by isolated thermophilic microorganism. The BUM strain was further tested for its ability in a soil composting system. Within a composting period of 42 days, removal of benzo[a]pyrene in the absence of BUM was 30.3%. Treatment with the inoculation of BUM significantly increased the removal of benzo[a]pyrene to 52.2%. Maximum zero-order degradation rates of benzo[a]pyrene by BUM in aqueous biodegradation experiment and soil composting system were 12.3 mg L-1 day -1 and 9.7 mg kg-1 day-1, respectively, which were significantly greater than most of the reported degradation rates by mesophiles. Experimental results affirmed that the strain BUM can effectively degrade benzo[a]pyrene under thermophilic condition.
AB - Two thermophilic bacterial strains, Bacillus subtilis BUM (BUM) and Mycobacterium vanbaalenii BU42 (BU42), were tested for their potential in biodegradation of benzo[a]pyrene. Neither BUM nor BU42 utilized benzo[a]pyrene as the sole substrate under thermophilic condition. In the presence of 50, 250, and 500 mg L-1 phenanthrene, the biodegradation of benzo[a]pyrene by BUM occurred and removals in 30 days were 14.8%, 38.8%, and 63.6%, respectively. This is the first report on the biodegradation of benzo[a]pyrene by isolated thermophilic microorganism. The BUM strain was further tested for its ability in a soil composting system. Within a composting period of 42 days, removal of benzo[a]pyrene in the absence of BUM was 30.3%. Treatment with the inoculation of BUM significantly increased the removal of benzo[a]pyrene to 52.2%. Maximum zero-order degradation rates of benzo[a]pyrene by BUM in aqueous biodegradation experiment and soil composting system were 12.3 mg L-1 day -1 and 9.7 mg kg-1 day-1, respectively, which were significantly greater than most of the reported degradation rates by mesophiles. Experimental results affirmed that the strain BUM can effectively degrade benzo[a]pyrene under thermophilic condition.
KW - Bacillus subtilis BUM
KW - biodegradation
KW - composting
KW - Mycobacterium vanbaalenii BU42
KW - polycyclic aromatic hydrocarbons
UR - http://www.scopus.com/inward/record.url?scp=78650134478&partnerID=8YFLogxK
U2 - 10.1089/ees.2010.0101
DO - 10.1089/ees.2010.0101
M3 - Journal article
AN - SCOPUS:78650134478
SN - 1092-8758
VL - 27
SP - 939
EP - 945
JO - Environmental Engineering Science
JF - Environmental Engineering Science
IS - 11
ER -