Rapid analysis of pseudolaric acids in Cortex Pseudolaricis and related medicinal products by high performance liquid chromatography

Quan Bin Han, Yue Keung Yip, Nian Yun Yang, Lina Wong, Chun Feng Qiao, Jing Zheng Song, Hillary Yiu, Hong Xi Xu*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

9 Citations (Scopus)


A simple, rapid, reverse-phase high performance liquid chromatographic method was developed for the quantitative analysis of pseudolaric acids in Cortex Pseudolaricis and its related medicinal products. With a C18 analytical column (4.6 mm × 150 mm i.d.), five pseudolaric acids, namely pseudolaric acids A-C, pseudolaric acid A-O-β-d-glucopyranoside and pseudolaric acid B-O-β-d-glucopyranoside, were well separated within 7 min. Acetonitrile and 0.10% acetic acid were used as the mobile phase in a gradient program. The UV detection wavelength was set at 260 nm. The detection limits and quantification limits ranged in 8.26-16.66 ng/ml and 27.54-55.53 ng/ml, respectively. The intra- and inter-day variations were less than 1% for all five compounds. The recovery of all spiked pseudolaric acids ranged from 99.1% to 101.9%. Compared to existing analytical methods, this new method not only used two more important chemical markers but also provided a fivefold reduction in analysis time. In addition, the extraction method of herb sample was also modified by an orthogonal array experiment on three variable parameters: extraction time, solvent volume, and extraction cycles. The optimized extraction method was much simpler and could be efficiently used to analyse large set of herbal materials and related medicinal products. Nineteen herb samples collected from different regions of China and five related products were examined with this new analytical method. The results showed that this method is effective in distinguishing adulterants and unqualified products.

Original languageEnglish
Pages (from-to)757-763
Number of pages7
Issue number4
Publication statusPublished - 15 Oct 2007

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • Cortex Pseudolaricis
  • HPLC
  • Pseudolaric acids
  • Pseudolarix kaempferi Gord.
  • Quality control


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