Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression

Wanrong Bao; Quanwei Zhang; Hongming Zheng; Lifeng Li; Man Liu; Huiyuan Cheng; Tinlong Wong; Ge Zhang; Aiping Lu; Zhaoxiang Bian; Diklung Ma; Chunghang Leung; Quanbin Han

doi:10.1016/j.ijbiomac.2021.05.120

Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression

Wanrong Bao, Quanwei Zhang, Hongming Zheng, Lifeng Li, Man Liu, Huiyuan Cheng, Tinlong Wong, Ge Zhang, Aiping Lu, Zhaoxiang Bian, Diklung Ma^*, Chunghang Leung^*, Quanbin Han^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Radix Astragali polysaccharide RAP has been reported to play a crucial role in hematopoiesis without a clear mechanism. In this study, RAP's effects to enhance the recovery of cyclophosphamide (Cy)-suppressed bone marrow and blood cells is confirmed in vivo first. Confocal micrographs demonstrated the interesting direct binding of FITC-RAP to hematopoietic stem cells (HSC) in bone marrow. RAP protects both mice and human HSC in terms of cell morphology, proliferation, and apoptosis. RNA-sequencing and shRNA approaches revealed FOS to be a key regulator in RAP's protection. These evidences provide an unreported mechanism that RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression.

Original language	English
Pages (from-to)	1715-1722
Number of pages	8
Journal	International Journal of Biological Macromolecules
Volume	183
DOIs	https://doi.org/10.1016/j.ijbiomac.2021.05.120
Publication status	Published - 31 Jul 2021

Scopus Subject Areas

Structural Biology
Biochemistry
Molecular Biology
Economics and Econometrics
Energy(all)

User-Defined Keywords

Astragalus polysaccharide
FOS
Hematopoietic stem cell
Myelosuppression
Protective effect

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10.1016/j.ijbiomac.2021.05.120

Cite this

Bao, W., Zhang, Q., Zheng, H., Li, L., Liu, M., Cheng, H., Wong, T., Zhang, G., Lu, A., Bian, Z., Ma, D., Leung, C., & Han, Q. (2021). Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression. International Journal of Biological Macromolecules, 183, 1715-1722. https://doi.org/10.1016/j.ijbiomac.2021.05.120

Bao, Wanrong ; Zhang, Quanwei ; Zheng, Hongming ; Li, Lifeng ; Liu, Man ; Cheng, Huiyuan ; Wong, Tinlong ; Zhang, Ge ; Lu, Aiping ; Bian, Zhaoxiang ; Ma, Diklung ; Leung, Chunghang ; Han, Quanbin. / Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression. In: International Journal of Biological Macromolecules. 2021 ; Vol. 183. pp. 1715-1722.

@article{9421ec34b3084f96a87fd8f73b8e3313,

title = "Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression",

abstract = "Radix Astragali polysaccharide RAP has been reported to play a crucial role in hematopoiesis without a clear mechanism. In this study, RAP's effects to enhance the recovery of cyclophosphamide (Cy)-suppressed bone marrow and blood cells is confirmed in vivo first. Confocal micrographs demonstrated the interesting direct binding of FITC-RAP to hematopoietic stem cells (HSC) in bone marrow. RAP protects both mice and human HSC in terms of cell morphology, proliferation, and apoptosis. RNA-sequencing and shRNA approaches revealed FOS to be a key regulator in RAP's protection. These evidences provide an unreported mechanism that RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression.",

keywords = "Astragalus polysaccharide, FOS, Hematopoietic stem cell, Myelosuppression, Protective effect",

author = "Wanrong Bao and Quanwei Zhang and Hongming Zheng and Lifeng Li and Man Liu and Huiyuan Cheng and Tinlong Wong and Ge Zhang and Aiping Lu and Zhaoxiang Bian and Diklung Ma and Chunghang Leung and Quanbin Han",

note = "Funding Information: This work was funded and supported by the HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09 ; the General Research Fund [ 12100615 , 22100014 , 12100818 ]; the UGC Research Matching Grant Scheme [ 2019-1-10 , 2019-1-14 , 2019-2-06 ]; the Health Medical Research Fund [ 11122531 , 14150521 , 17182681 ]; the National Natural Science Foundation of China [ 81473341 ]; the Science and Technology Project of Shenzhen [ JCYJ20160531193812867 ]; the Key-Area Research and Development Program of Guangdong Province [ 2020B1111110007 ]; Hong Kong Baptist University [RC-Start-up Grants, MPCF-001-2016/2017 , RC-IRMS/14-15/06 , FRG2/17-18/060 and FRG2/16-17/002 ]; and Vincent & Lily Woo Foundation . Publisher Copyright: {\textcopyright} 2021",

year = "2021",

month = jul,

day = "31",

doi = "10.1016/j.ijbiomac.2021.05.120",

language = "English",

volume = "183",

pages = "1715--1722",

journal = "International Journal of Biological Macromolecules",

issn = "0141-8130",

publisher = "Elsevier",

}

Bao, W, Zhang, Q, Zheng, H, Li, L, Liu, M, Cheng, H, Wong, T, Zhang, G , Lu, A , Bian, Z , Ma, D, Leung, C & Han, Q 2021, 'Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression', International Journal of Biological Macromolecules, vol. 183, pp. 1715-1722. https://doi.org/10.1016/j.ijbiomac.2021.05.120

Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression. / Bao, Wanrong; Zhang, Quanwei; Zheng, Hongming; Li, Lifeng; Liu, Man; Cheng, Huiyuan; Wong, Tinlong; Zhang, Ge ; Lu, Aiping ; Bian, Zhaoxiang ; Ma, Diklung; Leung, Chunghang; Han, Quanbin.

In: International Journal of Biological Macromolecules, Vol. 183, 31.07.2021, p. 1715-1722.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression

AU - Bao, Wanrong

AU - Zhang, Quanwei

AU - Zheng, Hongming

AU - Li, Lifeng

AU - Liu, Man

AU - Cheng, Huiyuan

AU - Wong, Tinlong

AU - Zhang, Ge

AU - Lu, Aiping

AU - Bian, Zhaoxiang

AU - Ma, Diklung

AU - Leung, Chunghang

AU - Han, Quanbin

N1 - Funding Information: This work was funded and supported by the HKSAR Innovation and Technology Fund (ITF), Tier 3, ITS/311/09 ; the General Research Fund [ 12100615 , 22100014 , 12100818 ]; the UGC Research Matching Grant Scheme [ 2019-1-10 , 2019-1-14 , 2019-2-06 ]; the Health Medical Research Fund [ 11122531 , 14150521 , 17182681 ]; the National Natural Science Foundation of China [ 81473341 ]; the Science and Technology Project of Shenzhen [ JCYJ20160531193812867 ]; the Key-Area Research and Development Program of Guangdong Province [ 2020B1111110007 ]; Hong Kong Baptist University [RC-Start-up Grants, MPCF-001-2016/2017 , RC-IRMS/14-15/06 , FRG2/17-18/060 and FRG2/16-17/002 ]; and Vincent & Lily Woo Foundation . Publisher Copyright: © 2021

PY - 2021/7/31

Y1 - 2021/7/31

N2 - Radix Astragali polysaccharide RAP has been reported to play a crucial role in hematopoiesis without a clear mechanism. In this study, RAP's effects to enhance the recovery of cyclophosphamide (Cy)-suppressed bone marrow and blood cells is confirmed in vivo first. Confocal micrographs demonstrated the interesting direct binding of FITC-RAP to hematopoietic stem cells (HSC) in bone marrow. RAP protects both mice and human HSC in terms of cell morphology, proliferation, and apoptosis. RNA-sequencing and shRNA approaches revealed FOS to be a key regulator in RAP's protection. These evidences provide an unreported mechanism that RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression.

AB - Radix Astragali polysaccharide RAP has been reported to play a crucial role in hematopoiesis without a clear mechanism. In this study, RAP's effects to enhance the recovery of cyclophosphamide (Cy)-suppressed bone marrow and blood cells is confirmed in vivo first. Confocal micrographs demonstrated the interesting direct binding of FITC-RAP to hematopoietic stem cells (HSC) in bone marrow. RAP protects both mice and human HSC in terms of cell morphology, proliferation, and apoptosis. RNA-sequencing and shRNA approaches revealed FOS to be a key regulator in RAP's protection. These evidences provide an unreported mechanism that RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression.

KW - Astragalus polysaccharide

KW - FOS

KW - Hematopoietic stem cell

KW - Myelosuppression

KW - Protective effect

UR - http://www.scopus.com/inward/record.url?scp=85106907787&partnerID=8YFLogxK

U2 - 10.1016/j.ijbiomac.2021.05.120

DO - 10.1016/j.ijbiomac.2021.05.120

M3 - Article

C2 - 34044030

AN - SCOPUS:85106907787

VL - 183

SP - 1715

EP - 1722

JO - International Journal of Biological Macromolecules

JF - International Journal of Biological Macromolecules

SN - 0141-8130

ER -

Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression

Abstract

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