Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression

Wanrong Bao, Quanwei Zhang, Hongming Zheng, Lifeng Li, Man Liu, Huiyuan Cheng, Tinlong Wong, Ge Zhang, Aiping Lu, Zhaoxiang Bian, Diklung Ma*, Chunghang Leung*, Quanbin Han*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Radix Astragali polysaccharide RAP has been reported to play a crucial role in hematopoiesis without a clear mechanism. In this study, RAP's effects to enhance the recovery of cyclophosphamide (Cy)-suppressed bone marrow and blood cells is confirmed in vivo first. Confocal micrographs demonstrated the interesting direct binding of FITC-RAP to hematopoietic stem cells (HSC) in bone marrow. RAP protects both mice and human HSC in terms of cell morphology, proliferation, and apoptosis. RNA-sequencing and shRNA approaches revealed FOS to be a key regulator in RAP's protection. These evidences provide an unreported mechanism that RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression.

Original languageEnglish
Pages (from-to)1715-1722
Number of pages8
JournalInternational Journal of Biological Macromolecules
Volume183
DOIs
Publication statusPublished - 31 Jul 2021

Scopus Subject Areas

  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Economics and Econometrics
  • Energy(all)

User-Defined Keywords

  • Astragalus polysaccharide
  • FOS
  • Hematopoietic stem cell
  • Myelosuppression
  • Protective effect

Fingerprint

Dive into the research topics of 'Radix Astragali polysaccharide RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression'. Together they form a unique fingerprint.

Cite this