Abstract
Radix Astragali polysaccharide RAP has been reported to play a crucial role in hematopoiesis without a clear mechanism. In this study, RAP's effects to enhance the recovery of cyclophosphamide (Cy)-suppressed bone marrow and blood cells is confirmed in vivo first. Confocal micrographs demonstrated the interesting direct binding of FITC-RAP to hematopoietic stem cells (HSC) in bone marrow. RAP protects both mice and human HSC in terms of cell morphology, proliferation, and apoptosis. RNA-sequencing and shRNA approaches revealed FOS to be a key regulator in RAP's protection. These evidences provide an unreported mechanism that RAP directly protects hematopoietic stem cells from chemotherapy-induced myelosuppression by increasing FOS expression.
Original language | English |
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Pages (from-to) | 1715-1722 |
Number of pages | 8 |
Journal | International Journal of Biological Macromolecules |
Volume | 183 |
DOIs | |
Publication status | Published - 31 Jul 2021 |
Scopus Subject Areas
- Structural Biology
- Biochemistry
- Molecular Biology
- Economics and Econometrics
- Energy(all)
User-Defined Keywords
- Astragalus polysaccharide
- FOS
- Hematopoietic stem cell
- Myelosuppression
- Protective effect