TY - JOUR
T1 - Quantum dot-ruthenium complex dyads
T2 - Recognition of double-strand DNA through dual-color fluorescence detection
AU - Zhao, Dan
AU - Chan, W. H.
AU - He, Zhike
AU - Qiu, Ting
N1 - This work was supported by grants from the Science Faculty of Hong Kong Baptist University (Chemical and Bio-chemical Sensing), Science Fund for Creative Research Groups of NSFC (Grant 20621502), the National Key Scientific Program-Nanoscience and Nanotechnology (Grant No. 2006CB933103), and the 973 Program (Grant No. 2007CB714507).
PY - 2009/5/1
Y1 - 2009/5/1
N2 - We have developed a new fluorescent ensemble probe comprising an ionic conjugate between water-soluble thioglycolic acid (TGA) capped CdTe quantum dots (QDs) and Ru(bpy)2(dppx)2+ for the dual-color detection of complementary double-stranded DNAs (dsDNA). To provide the platform for DNA detection, the Rucomplex was first employed as an effective fluorescence quencher to TGA capped QDs via photoinduced electron transfer process. Because of its strong binding affinity with Ru(bpy)2(dppx)2+, complementary dsDNA can break up the low fluoresced ionic ensemble, set free the luminescent QDs, and concomitantly generate the Ru(bpy)2(dppx) 2+ intercalated DNA complex. Thus, the recognition of dsDNA by Ru(bpy)2(dppx)2+ can be realized via both the restoration of QDs fluorescence and the emergence of a new fluorescence emission signal of the quencher-substrate at 609 nm, while single-stranded DNA, ribonucleic acid, bovine albumin serum, and biological relevant metal ions cannot produce the similar results. Therefore, a simple, fast, sensitive, and highly selective assay for dsDNA has been realized.
AB - We have developed a new fluorescent ensemble probe comprising an ionic conjugate between water-soluble thioglycolic acid (TGA) capped CdTe quantum dots (QDs) and Ru(bpy)2(dppx)2+ for the dual-color detection of complementary double-stranded DNAs (dsDNA). To provide the platform for DNA detection, the Rucomplex was first employed as an effective fluorescence quencher to TGA capped QDs via photoinduced electron transfer process. Because of its strong binding affinity with Ru(bpy)2(dppx)2+, complementary dsDNA can break up the low fluoresced ionic ensemble, set free the luminescent QDs, and concomitantly generate the Ru(bpy)2(dppx) 2+ intercalated DNA complex. Thus, the recognition of dsDNA by Ru(bpy)2(dppx)2+ can be realized via both the restoration of QDs fluorescence and the emergence of a new fluorescence emission signal of the quencher-substrate at 609 nm, while single-stranded DNA, ribonucleic acid, bovine albumin serum, and biological relevant metal ions cannot produce the similar results. Therefore, a simple, fast, sensitive, and highly selective assay for dsDNA has been realized.
UR - http://www.scopus.com/inward/record.url?scp=66149120243&partnerID=8YFLogxK
U2 - 10.1021/ac9000892
DO - 10.1021/ac9000892
M3 - Journal article
C2 - 19351144
AN - SCOPUS:66149120243
SN - 0003-2700
VL - 81
SP - 3537
EP - 3543
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 9
ER -