Quantitative metabolic network profiling of Escherichia coli: An overview of analytical methods for measurement of intracellular metabolites

Yang Shen, Tohidi Fatemeh, Lei Han TANG, Zongwei CAI*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

14 Citations (Scopus)

Abstract

Understanding the mechanism of metabolic network response to extracellular changes has become one of the primary objectives of systems biology. Quantitative metabolic network profiling of model organisms such as E. coli is the principal approach. Well-designed analytical protocols for the determination of intracellular metabolites may provide a crucial premise to quantitative profiling of cellular metabolism. This article reviews and evaluates the existing methods for determining intracellular metabolites of E. coli in terms of sample preparation and mass spectrometry (MS) analysis. Critical issues including the variation of metabolic status and interference of external pools during cell sampling, sample loss during quenching procedures, degradation of metabolites and efficiency of extraction, and matrix effects in separation and quantification are discussed. Moreover, suggestions are provided to solve the analytical problems based on the results from the recent studies in order to establish a precise and reliable analytical platform for the intracellular metabolites.

Original languageEnglish
Pages (from-to)141-150
Number of pages10
JournalTrAC - Trends in Analytical Chemistry
Volume75
DOIs
Publication statusPublished - 1 Jan 2016

Scopus Subject Areas

  • Analytical Chemistry
  • Spectroscopy

User-Defined Keywords

  • Escherichia coli
  • Intracellular metabolites
  • Mass spectrometry
  • Metabolic network profiling
  • Sample preparation

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