TY - JOUR
T1 - Quantitative determination of four diterpenoids in Radix Salviae Miltiorrhizae using LC-MS-MS
AU - Hu, Ping
AU - Luo, Guo An
AU - ZHAO, Zhongzhen
AU - JIANG, Zhi Hong
N1 - Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2005/6
Y1 - 2005/6
N2 - An HPLC-ESI-MS-MS method has been developed for the quantitative determination of four diterpenoids, dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA in Radix Salviae Miltiorrhizae (RSM, the root of Salvia miltiorrhiza BGE.). The diterpenoids were chromatographically separated on a C18 HPLC column, and the quantification of these diterpenoids was based on the fragments of [M+H]+ under collision-activated conditions and in Selected Reaction Monitioring (SRM) mode. The quantitative method was validated, and the mean recovery rates from fortified samples (n=5) of dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone UA were 95.0%, 97.2%, 93.1%, and 95.9% with variation coefficient of 6.0%, 4.3%, 3.7%, and 4.2%, respectively. The established method was successfully applied to the quality assessment of seven batches of RSM samples collected from different regions of China.
AB - An HPLC-ESI-MS-MS method has been developed for the quantitative determination of four diterpenoids, dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone IIA in Radix Salviae Miltiorrhizae (RSM, the root of Salvia miltiorrhiza BGE.). The diterpenoids were chromatographically separated on a C18 HPLC column, and the quantification of these diterpenoids was based on the fragments of [M+H]+ under collision-activated conditions and in Selected Reaction Monitioring (SRM) mode. The quantitative method was validated, and the mean recovery rates from fortified samples (n=5) of dihydrotanshinone I, cryptotanshinone, tanshinone I, and tanshinone UA were 95.0%, 97.2%, 93.1%, and 95.9% with variation coefficient of 6.0%, 4.3%, 3.7%, and 4.2%, respectively. The established method was successfully applied to the quality assessment of seven batches of RSM samples collected from different regions of China.
KW - Diterpenoid
KW - HPLC-ESI-MS-MS
KW - Quantitative analysis
KW - Salvia miltiorrhiza
KW - Tanshinone
UR - http://www.scopus.com/inward/record.url?scp=21144444351&partnerID=8YFLogxK
U2 - 10.1248/cpb.53.705
DO - 10.1248/cpb.53.705
M3 - Journal article
C2 - 15930789
AN - SCOPUS:21144444351
SN - 0009-2363
VL - 53
SP - 705
EP - 709
JO - Chemical and Pharmaceutical Bulletin
JF - Chemical and Pharmaceutical Bulletin
IS - 6
ER -