TY - JOUR
T1 - Quantifying silver nanoparticle association and elemental content in single cells using dual mass mode in quadrupole-based inductively coupled plasma-mass spectrometry
AU - Lum, Judy Tsz Shan
AU - Leung, Kelvin Sze Yin
N1 - Funding Information:
The authors thank Dr. Olga Borovinskaya, Dr. Martin Rittner and Dr. Martin Tanner (TOFWERK, AG, Thun, Switzerland) for their assistance in ICP-TOFMS analysis and data processing. K. S. -Y. Leung is grateful for funding support from the Inter-institutional Collaborative Research Scheme (RC-ICRS/16-17/02B), Inter-disciplinary Research Matching Scheme (RC-IRMS/16-17/01A) and Partner State Key Laboratory of Environmental and Biological Analysis (SKLP-1415-P006), Hong Kong Baptist University. Kelvin S. -Y. Leung acknowledges the Science, Technology and Innovation Commission of Shenzhen (JCYJ20170817173243420) for funding support.
Publisher copyright:
© 2019 Elsevier B.V.
PY - 2019/7/11
Y1 - 2019/7/11
N2 - In this study, a method of simultaneous dual mass detection for single cell analysis by quadrupole-based ICP-MS (ICP-QMS) is proposed. The method shows potential for use in quantitative investigations of nanoparticle association and elemental composition of cells. Dual mass detection had been attempted in the analysis of two-element core-shell nanoparticles and in isotope dilution analysis. In this method the detector switches between two selected masses during the analysis. Dual mass mode eliminates the discrepancies in signal that can occur due to sample instability or fluctuation in sample uptake when two masses are analysed sequentially by conventional single cell analysis (SP mode). Preliminary tests showed that using an Mg spike as marker of cells in dual mass mode was feasible for the quantification of cells. The method showed good linearity and a reproducible detection rate, and the results were comparable to the SP mode. The approach was then employed with algal cells exposed to silver nanoparticles (AgNP), to study on the Ag-associated cells and AgNP by monitoring the Ag and Mg signal in one analytical run. Finally, Mg and Mn were detected, and then quantified using the same approach to evaluate the elemental composition and correlation between different elements of the exposed cells. It is believed that this dual mass approach can extend the capability of ICP-QMS for multi-elemental detection at the single cell level, representing an enormous potential for size characterization, quantification and elemental composition evaluation in single cell (particle) analysis.
AB - In this study, a method of simultaneous dual mass detection for single cell analysis by quadrupole-based ICP-MS (ICP-QMS) is proposed. The method shows potential for use in quantitative investigations of nanoparticle association and elemental composition of cells. Dual mass detection had been attempted in the analysis of two-element core-shell nanoparticles and in isotope dilution analysis. In this method the detector switches between two selected masses during the analysis. Dual mass mode eliminates the discrepancies in signal that can occur due to sample instability or fluctuation in sample uptake when two masses are analysed sequentially by conventional single cell analysis (SP mode). Preliminary tests showed that using an Mg spike as marker of cells in dual mass mode was feasible for the quantification of cells. The method showed good linearity and a reproducible detection rate, and the results were comparable to the SP mode. The approach was then employed with algal cells exposed to silver nanoparticles (AgNP), to study on the Ag-associated cells and AgNP by monitoring the Ag and Mg signal in one analytical run. Finally, Mg and Mn were detected, and then quantified using the same approach to evaluate the elemental composition and correlation between different elements of the exposed cells. It is believed that this dual mass approach can extend the capability of ICP-QMS for multi-elemental detection at the single cell level, representing an enormous potential for size characterization, quantification and elemental composition evaluation in single cell (particle) analysis.
KW - Cell quantification
KW - Elemental composition
KW - Inductively coupled plasma time-of-flight-mass spectrometry
KW - Inductively coupled plasma-quadrupole-mass spectrometry
KW - Nanoparticles
KW - Single cell analysis
UR - http://www.scopus.com/inward/record.url?scp=85062674736&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2019.02.042
DO - 10.1016/j.aca.2019.02.042
M3 - Journal article
C2 - 30926039
AN - SCOPUS:85062674736
SN - 0003-2670
VL - 1061
SP - 50
EP - 59
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
ER -