Quantification of zeaxanthin dipalmitate and total carotenoids in Lycium fruits (Fructus Lycii)

Yong Peng; Chen Ma; Yawei Li; Kelvin S Y LEUNG; Zhi Hong JIANG; Zhongzhen ZHAO

doi:10.1007/s11130-005-9550-5

Quantification of zeaxanthin dipalmitate and total carotenoids in Lycium fruits (Fructus Lycii)

Yong Peng, Chen Ma, Yawei Li, Kelvin S Y LEUNG, Zhi Hong JIANG^*, Zhongzhen ZHAO

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

139 Citations (Scopus)

Abstract

An HPLC-DAD method has been developed to quantitatively analyze for the content of zeaxanthin dipalmitate, a major carotenoid in Fructus Lycii, in different species of the genus Lycium. Determination was performed using an Alltima C18 column with the mobile phase consisting of acetonitrile and dichloromethane (42:58). The total contents of carotenoids in these samples were also determined by using UV spectrophotometric assay. Total carotenoid concentrations of different Fructus Lycii are within the range of 0.03-0.5%. Zeaxanthin dipalmitate is a predominant carotenoid. comprising 31-56% of the total carotenoids in Fructus Lycii. This study is the first systematic quantification of the carotenoids in the fruits of different Lycium species. The results demonstrated that these methods are reliable and facile techniques for rapid analysis of carotenoids for crude drug and plant-derived food supplements.

Original language	English
Pages (from-to)	161-164
Number of pages	4
Journal	Plant Foods for Human Nutrition
Volume	60
Issue number	4
DOIs	https://doi.org/10.1007/s11130-005-9550-5
Publication status	Published - Dec 2005

Scopus Subject Areas

Food Science
Chemistry (miscellaneous)

User-Defined Keywords

Fructus Lycii
HPLC chromatography
Lycium
Total carotenoids
Zeaxanthin dipalmitate

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10.1007/s11130-005-9550-5

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title = "Quantification of zeaxanthin dipalmitate and total carotenoids in Lycium fruits (Fructus Lycii)",

abstract = "An HPLC-DAD method has been developed to quantitatively analyze for the content of zeaxanthin dipalmitate, a major carotenoid in Fructus Lycii, in different species of the genus Lycium. Determination was performed using an Alltima C18 column with the mobile phase consisting of acetonitrile and dichloromethane (42:58). The total contents of carotenoids in these samples were also determined by using UV spectrophotometric assay. Total carotenoid concentrations of different Fructus Lycii are within the range of 0.03-0.5%. Zeaxanthin dipalmitate is a predominant carotenoid. comprising 31-56% of the total carotenoids in Fructus Lycii. This study is the first systematic quantification of the carotenoids in the fruits of different Lycium species. The results demonstrated that these methods are reliable and facile techniques for rapid analysis of carotenoids for crude drug and plant-derived food supplements.",

keywords = "Fructus Lycii, HPLC chromatography, Lycium, Total carotenoids, Zeaxanthin dipalmitate",

author = "Yong Peng and Chen Ma and Yawei Li and LEUNG, {Kelvin S Y} and JIANG, {Zhi Hong} and Zhongzhen ZHAO",

note = "Funding Information: This work was financially supported by the Faculty Research Grants from Hong Kong Baptist University.",

year = "2005",

month = dec,

doi = "10.1007/s11130-005-9550-5",

language = "English",

volume = "60",

pages = "161--164",

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T1 - Quantification of zeaxanthin dipalmitate and total carotenoids in Lycium fruits (Fructus Lycii)

AU - Peng, Yong

AU - Ma, Chen

AU - Li, Yawei

AU - LEUNG, Kelvin S Y

AU - JIANG, Zhi Hong

AU - ZHAO, Zhongzhen

N1 - Funding Information: This work was financially supported by the Faculty Research Grants from Hong Kong Baptist University.

PY - 2005/12

Y1 - 2005/12

N2 - An HPLC-DAD method has been developed to quantitatively analyze for the content of zeaxanthin dipalmitate, a major carotenoid in Fructus Lycii, in different species of the genus Lycium. Determination was performed using an Alltima C18 column with the mobile phase consisting of acetonitrile and dichloromethane (42:58). The total contents of carotenoids in these samples were also determined by using UV spectrophotometric assay. Total carotenoid concentrations of different Fructus Lycii are within the range of 0.03-0.5%. Zeaxanthin dipalmitate is a predominant carotenoid. comprising 31-56% of the total carotenoids in Fructus Lycii. This study is the first systematic quantification of the carotenoids in the fruits of different Lycium species. The results demonstrated that these methods are reliable and facile techniques for rapid analysis of carotenoids for crude drug and plant-derived food supplements.

AB - An HPLC-DAD method has been developed to quantitatively analyze for the content of zeaxanthin dipalmitate, a major carotenoid in Fructus Lycii, in different species of the genus Lycium. Determination was performed using an Alltima C18 column with the mobile phase consisting of acetonitrile and dichloromethane (42:58). The total contents of carotenoids in these samples were also determined by using UV spectrophotometric assay. Total carotenoid concentrations of different Fructus Lycii are within the range of 0.03-0.5%. Zeaxanthin dipalmitate is a predominant carotenoid. comprising 31-56% of the total carotenoids in Fructus Lycii. This study is the first systematic quantification of the carotenoids in the fruits of different Lycium species. The results demonstrated that these methods are reliable and facile techniques for rapid analysis of carotenoids for crude drug and plant-derived food supplements.

KW - Fructus Lycii

KW - HPLC chromatography

KW - Lycium

KW - Total carotenoids

KW - Zeaxanthin dipalmitate

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DO - 10.1007/s11130-005-9550-5

M3 - Journal article

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AN - SCOPUS:30144443594

SN - 0921-9668

VL - 60

SP - 161

EP - 164

JO - Plant Foods for Human Nutrition

JF - Plant Foods for Human Nutrition

IS - 4

ER -

Quantification of zeaxanthin dipalmitate and total carotenoids in Lycium fruits (Fructus Lycii)

Abstract

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