Quantification and stability studies on the flavonoids of Radix hedysari

Yi Liu, Hubiao CHEN, Yu Ying Zhao, Bin Wang, Qing Ying Zhang*, Lei Zhang, Pengfei Tu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Radix hedysari is not only a restorative food but also a famous Traditional Chinese Medicine. In this study, a simple, reliable, and reproducible high-performance liquid chromatography-ultraviolet method was developed for the first time to determine the true contents of five major flavoniods, naringenin-7-O-α-L-rhamanopyranosyl-(1→2)-β-D-glucopyranoside, ononin, formononetin, medicarpin, and an unstable flavonoid malonate (formononetin-7-O-β-D-glucoside-6″-O-malonate, FGM), and the stability of FGM was also investigated. Flavoniods were selected as chemical markers because they have appeared as one of the major bioactive compound groups in Radix hedysari. The stability results of FGM at different pH showed that it remained relatively stable in acidic aqueous methanol of pH 3. The HPLC assay with an improved sample preparation scheme can be readily utilized as a suitable "chemical" quality control method for Radix hedysari.

Original languageEnglish
Pages (from-to)6634-6639
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume54
Issue number18
DOIs
Publication statusPublished - 6 Sep 2006

Scopus Subject Areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

User-Defined Keywords

  • Flavonoids
  • Formononetin-7-O-β-D-glucoside-6″-O-malonate
  • HPLC-UV
  • Radix hedysari

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