Quality assessment of Cortex phellodendri by high-performance liquid chromatography coupled with electrospray ionization mass spectrometry

Yong Mei Hu, Guang Hai Su, Stephen Cho Wing Sze, Wencai Ye, Yao Tong*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

A simple method based on liquid chromatography coupled with diode array detection and electrospray ionization mass spectrometry (LC-DAD-ESI-MS) was developed for the quality assessment of Cortex Phellodendri (CP), which was mainly derived from two species of Phellodendron chinense Schneid and Phellodendron amurense Rupr. Total 41 compounds, including 14 phenols, 24 alkaloids and three liminoidal triterpenes were identified or tentatively characterized from the 75% methanol extract of CP samples by online ESI-MSn fragmentation and UV spectra analysis. Among them, two phenols and six alkaloids were simultaneously quantified using HPLC-DAD method. The validated HPLC-DAD method showed a good linearity, precision, repeatability and accuracy for the quantification of eight marker compounds. Furthermore, the plausible fragmentation pathway of the representative compounds were proposed in the present study. The differences of the chemical constituents content and the comprehensive HPLC profiles between the two CP species using LC-DAD-ESI-MS method are reported for the first time, indicating that the CP drugs from different resources should be used separately in the clinic.
Original languageEnglish
Pages (from-to)438-453
Number of pages16
JournalBiomedical Chromatography
Volume24
Issue number4
Early online date25 Aug 2009
DOIs
Publication statusPublished - Apr 2010
Externally publishedYes

User-Defined Keywords

  • Cortex phellodendri
  • HPLC-UV-ESI/MS
  • Limonoidal triterpenes
  • Phenols
  • Quality assessment
  • Quaternary isoquinoline alkaloids
  • Traditional herbal medicine (THM)

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