Qualitative and quantitative analyses of nucleosides and nucleobases in Ganoderma spp. by HPLC-DAD-MS

J. L. Gao, Kelvin S Y LEUNG, Y. T. Wang, C. M. Lai, S. P. Li, L. F. Hu, G. H. Lu, Zhi Hong Jiang, Zhiling Yu*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

83 Citations (Scopus)


A high-performance liquid chromatography-diode array detector-mass spectrometry (HPLC-DAD-MS) analytical method was developed for detection of the nucleosides and nucleobases in two species of Lingzhi, the dried sporophore of Ganoderma lucidum and G. sinense. The method, combining advantages of both DAD and MS, was successfully used to qualitatively identify for six nucleosides namely, adenosine, cytidine, guanosine, inosine, thymidine, uridine and five nucleobases namely, adenine, guanine, hypoxanthine, thymine and uracil in Lingzhi samples. Quantitative analyses showed that uridine was the most abundant nucleoside in these Lingzhi samples and the contents of nine target analytes were found to be different in pileus and stipes of the fruiting bodies and among the different species of G. spp. The established method might apply as an alternative approach for the quality assessment of Lingzhi.

Original languageEnglish
Pages (from-to)807-811
Number of pages5
JournalJournal of Pharmaceutical and Biomedical Analysis
Issue number3 SPEC. ISS.
Publication statusPublished - 27 Jul 2007

Scopus Subject Areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

User-Defined Keywords

  • G. sinense
  • Ganoderma lucidum
  • Nucleobase
  • Nucleoside
  • Quantitative analysis


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