Abstract
A high-performance liquid chromatography-diode array detector-mass spectrometry (HPLC-DAD-MS) analytical method was developed for detection of the nucleosides and nucleobases in two species of Lingzhi, the dried sporophore of Ganoderma lucidum and G. sinense. The method, combining advantages of both DAD and MS, was successfully used to qualitatively identify for six nucleosides namely, adenosine, cytidine, guanosine, inosine, thymidine, uridine and five nucleobases namely, adenine, guanine, hypoxanthine, thymine and uracil in Lingzhi samples. Quantitative analyses showed that uridine was the most abundant nucleoside in these Lingzhi samples and the contents of nine target analytes were found to be different in pileus and stipes of the fruiting bodies and among the different species of G. spp. The established method might apply as an alternative approach for the quality assessment of Lingzhi.
| Original language | English |
|---|---|
| Pages (from-to) | 807-811 |
| Number of pages | 5 |
| Journal | Journal of Pharmaceutical and Biomedical Analysis |
| Volume | 44 |
| Issue number | 3 SPEC. ISS. |
| DOIs | |
| Publication status | Published - 27 Jul 2007 |
Scopus Subject Areas
- Analytical Chemistry
- Pharmaceutical Science
- Drug Discovery
- Spectroscopy
- Clinical Biochemistry
User-Defined Keywords
- G. sinense
- Ganoderma lucidum
- HPLC-DAD-MS
- Nucleobase
- Nucleoside
- Quantitative analysis