Putrescine biosynthesis in Tetrahymena thermophila.

K. M. Yao*, David W F FONG, S. F. Ng

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

The putrescine-biosynthesis pathway in Tetrahymena thermophila was delineated by studying crude extracts prepared from exponentially growing cultures. A pyridoxal phosphate-stimulated ornithine decarboxylase activity competitively inhibited by putrescine was detected. CO2 was also liberated from L-arginine, but analyses by t.l.c. and enzyme studies suggested that the activity was not due to arginine decarboxylase, nor could enzyme activities converting agmatine into putrescine be detected. We conclude that the decarboxylation of L-ornithine is probably the only major route for putrescine biosynthesis in this organism during exponential growth.

Original languageEnglish
Pages (from-to)679-684
Number of pages6
JournalBiochemical Journal
Volume222
Issue number3
DOIs
Publication statusPublished - 15 Sep 1984

Scopus Subject Areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint

Dive into the research topics of 'Putrescine biosynthesis in Tetrahymena thermophila.'. Together they form a unique fingerprint.

Cite this