Purification and partial structural characterization of a complement fixating polysaccharide from rhizomes of Ligusticum chuanxiong

Yuan Feng Zou; Yu Ping Fu; Xing Fu Chen; Ingvild Austarheim; Kari Tvete Inngjerdingen; Chao Huang; Lemlem Dugassa Eticha; Xu Song; Lixia Li; Bin Feng; Chang Liang He; Zhong Qiong Yin; Berit Smestad Paulsen; Simon Q B HAN

doi:10.3390/molecules22020287

Purification and partial structural characterization of a complement fixating polysaccharide from rhizomes of Ligusticum chuanxiong

Yuan Feng Zou
, Yu Ping Fu
, Xing Fu Chen^*
, Ingvild Austarheim
, Kari Tvete Inngjerdingen
, Chao Huang
, Lemlem Dugassa Eticha
, Xu Song
, Lixia Li
, Bin Feng
, Chang Liang He
, Zhong Qiong Yin
, Berit Smestad Paulsen
, Simon Q B HAN

^*Corresponding author for this work

Chinese Medicine - Teaching and Research Division

Research output: Contribution to journal › Journal article › peer-review

30 Citations (Scopus)

Abstract

Rhizome of Ligusticum chuanxiong is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100°C water extracts of L. chuangxiong rhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH₅₀ of 26.3 ± 2.2 μg/mL, and thus has potential as a natural immunomodulator.

Original language	English
Article number	287
Journal	Molecules
Volume	22
Issue number	2
DOIs	https://doi.org/10.3390/molecules22020287
Publication status	Published - Feb 2017

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This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

Complement system
Ligusticum chuanxiong
Pectic polysaccharide
Polysaccharides
Purification

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10.3390/molecules22020287

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Zou, Y. F., Fu, Y. P., Chen, X. F., Austarheim, I., Inngjerdingen, K. T., Huang, C., Eticha, L. D., Song, X., Li, L., Feng, B., He, C. L., Yin, Z. Q., Paulsen, B. S., & HAN, S. Q. B. (2017). Purification and partial structural characterization of a complement fixating polysaccharide from rhizomes of Ligusticum chuanxiong. Molecules, 22(2), Article 287. https://doi.org/10.3390/molecules22020287

@article{865d460f5be64f44af6139f893935fa8,

title = "Purification and partial structural characterization of a complement fixating polysaccharide from rhizomes of Ligusticum chuanxiong",

abstract = "Rhizome of Ligusticum chuanxiong is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100°C water extracts of L. chuangxiong rhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH50 of 26.3 ± 2.2 μg/mL, and thus has potential as a natural immunomodulator.",

keywords = "Complement system, Ligusticum chuanxiong, Pectic polysaccharide, Polysaccharides, Purification",

author = "Zou, \{Yuan Feng\} and Fu, \{Yu Ping\} and Chen, \{Xing Fu\} and Ingvild Austarheim and Inngjerdingen, \{Kari Tvete\} and Chao Huang and Eticha, \{Lemlem Dugassa\} and Xu Song and Lixia Li and Bin Feng and He, \{Chang Liang\} and Yin, \{Zhong Qiong\} and Paulsen, \{Berit Smestad\} and HAN, \{Simon Q B\}",

note = "Funding Information: We acknowledge the financial supported by Huichuntang Pharmaceutical Co., Ltd. and Science \& Technological Department of Sichuan Province, China (2011NZ0098-12-01), and Scientific Research Start-up Funding from Sichuan Agricultural University. We are also grateful for valuable NMR discussions with Frode Rise, Chemistry department, University of Oslo, Norway.",

year = "2017",

month = feb,

doi = "10.3390/molecules22020287",

language = "English",

volume = "22",

journal = "Molecules",

issn = "1420-3049",

publisher = "MDPI",

number = "2",

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TY - JOUR

T1 - Purification and partial structural characterization of a complement fixating polysaccharide from rhizomes of Ligusticum chuanxiong

AU - Zou, Yuan Feng

AU - Fu, Yu Ping

AU - Chen, Xing Fu

AU - Austarheim, Ingvild

AU - Inngjerdingen, Kari Tvete

AU - Huang, Chao

AU - Eticha, Lemlem Dugassa

AU - Song, Xu

AU - Li, Lixia

AU - Feng, Bin

AU - He, Chang Liang

AU - Yin, Zhong Qiong

AU - Paulsen, Berit Smestad

AU - HAN, Simon Q B

N1 - Funding Information: We acknowledge the financial supported by Huichuntang Pharmaceutical Co., Ltd. and Science & Technological Department of Sichuan Province, China (2011NZ0098-12-01), and Scientific Research Start-up Funding from Sichuan Agricultural University. We are also grateful for valuable NMR discussions with Frode Rise, Chemistry department, University of Oslo, Norway.

PY - 2017/2

Y1 - 2017/2

N2 - Rhizome of Ligusticum chuanxiong is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100°C water extracts of L. chuangxiong rhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH50 of 26.3 ± 2.2 μg/mL, and thus has potential as a natural immunomodulator.

AB - Rhizome of Ligusticum chuanxiong is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100°C water extracts of L. chuangxiong rhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH50 of 26.3 ± 2.2 μg/mL, and thus has potential as a natural immunomodulator.

KW - Complement system

KW - Ligusticum chuanxiong

KW - Pectic polysaccharide

KW - Polysaccharides

KW - Purification

UR - https://www.scopus.com/pages/publications/85013174218

U2 - 10.3390/molecules22020287

DO - 10.3390/molecules22020287

M3 - Journal article

C2 - 28216596

AN - SCOPUS:85013174218

SN - 1420-3049

VL - 22

JO - Molecules

JF - Molecules

IS - 2

M1 - 287

ER -

Purification and partial structural characterization of a complement fixating polysaccharide from rhizomes of Ligusticum chuanxiong

Abstract

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