TY - JOUR
T1 - Purification and partial structural characterization of a complement fixating polysaccharide from rhizomes of Ligusticum chuanxiong
AU - Zou, Yuan Feng
AU - Fu, Yu Ping
AU - Chen, Xing Fu
AU - Austarheim, Ingvild
AU - Inngjerdingen, Kari Tvete
AU - Huang, Chao
AU - Eticha, Lemlem Dugassa
AU - Song, Xu
AU - Li, Lixia
AU - Feng, Bin
AU - He, Chang Liang
AU - Yin, Zhong Qiong
AU - Paulsen, Berit Smestad
AU - HAN, Simon Q B
N1 - Funding Information:
We acknowledge the financial supported by Huichuntang Pharmaceutical Co., Ltd. and Science & Technological Department of Sichuan Province, China (2011NZ0098-12-01), and Scientific Research Start-up Funding from Sichuan Agricultural University. We are also grateful for valuable NMR discussions with Frode Rise, Chemistry department, University of Oslo, Norway.
PY - 2017/2
Y1 - 2017/2
N2 - Rhizome of Ligusticum chuanxiong is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100°C water extracts of L. chuangxiong rhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH50 of 26.3 ± 2.2 μg/mL, and thus has potential as a natural immunomodulator.
AB - Rhizome of Ligusticum chuanxiong is an effective medical plant, which has been extensively applied for centuries in migraine and cardiovascular diseases treatment in China. Polysaccharides from this plant have been shown to have interesting bioactivities, but previous studies have only been performed on the neutral polysaccharides. In this study, LCP-I-I, a pectic polysaccharide fraction, was obtained from the 100°C water extracts of L. chuangxiong rhizomes and purified by diethylaminethyl (DEAE) sepharose anion exchange chromatography and gel filtration. Monosaccharide analysis and linkage determination in addition to Fourier transform infrared (FT-IR) spectrometer and Nuclear magnetic resonance (NMR) spectrum, indicated that LCP-I-I is a typical pectic polysaccharide, with homo-galacturonan and rhamnogalacturonan type I regions and arabinogalactan type I and type II (AG-I/AG-II) side chains. LCP-I-I exhibited potent complement fixation activity, ICH50 of 26.3 ± 2.2 μg/mL, and thus has potential as a natural immunomodulator.
KW - Complement system
KW - Ligusticum chuanxiong
KW - Pectic polysaccharide
KW - Polysaccharides
KW - Purification
UR - http://www.scopus.com/inward/record.url?scp=85013174218&partnerID=8YFLogxK
U2 - 10.3390/molecules22020287
DO - 10.3390/molecules22020287
M3 - Journal article
C2 - 28216596
AN - SCOPUS:85013174218
SN - 1420-3049
VL - 22
JO - Molecules
JF - Molecules
IS - 2
M1 - 287
ER -
